Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice
Abou Zaid, O., AbdEl Maksoud, H., Badwi, A., Hussien, M., EL-Shiekha, M. (2015). Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice. EKB Journal Management System, 28(1), 98-108. doi: 10.21608/bvmj.2015.32661
Omayma A.R. Abou Zaid; Hussein AbdEl Maksoud; Abdel Fatah M. Badwi; Mohamed A. Hussien; Mohammed F. EL-Shiekha. "Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice". EKB Journal Management System, 28, 1, 2015, 98-108. doi: 10.21608/bvmj.2015.32661
Abou Zaid, O., AbdEl Maksoud, H., Badwi, A., Hussien, M., EL-Shiekha, M. (2015). 'Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice', EKB Journal Management System, 28(1), pp. 98-108. doi: 10.21608/bvmj.2015.32661
Abou Zaid, O., AbdEl Maksoud, H., Badwi, A., Hussien, M., EL-Shiekha, M. Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice. EKB Journal Management System, 2015; 28(1): 98-108. doi: 10.21608/bvmj.2015.32661

Molecular study on the potential therapeutic effect of novel nanocompsite on cancerous tumor bearing mice

Benha Veterinary Medical Journal
Article 11, Volume 28, Issue 1, March 2015, Page 98-108  XML PDF (508.43 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2015.32661
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Authors
Omayma A.R. Abou Zaid; Hussein AbdEl Maksoud; Abdel Fatah M. Badwi; Mohamed A. Hussien; Mohammed F. EL-Shiekha
Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Qalioubeya, Egypt
Abstract
Nanoparticles are making significant contributions to the development of new approaches of drug
delivery in cancer and can provide a platform for combined therapeutics with subsequent monitoring of
response. Basic curcumin and zinc oxide (ZnO) nanocompsite modified with vitamin C and CTAB have
been exert chemopreventative activity against cancer in mice animal model. This study was carried out
on 80 mice and were divided into four groups, each groups have (20 mice) Group 1: NTBM (Negative
control) .Group 2: TBM- (Positive control). Group 3: NTBM-treated with nanocompsite orally (1.850
g/kg /day) for 6 weeks. Group 4: TBM-treated with nanocompsite orally (1.850 g/kg /day) for 6 weeks.
Liver tissues sample were collected from all mice by decapitation after 2, 4 and 6 weeks from the onset
of treatment, then obtained in dry and clean tubes then kept in a deep freeze at -20 oC and processed,
total RNA isolation and quantitative real time PCR analysis for apoptotic marker (P53- gene expression),
apoptotic DNA ladder assay and estimation of protein. We observed that nanocompsite have distinct
effects on liver cell viability via killing cancer cells, while posing no effect on normal cells
(hepatocytes). The marked difference in cytotoxicity between cancer cells and normal cells suggests an
exciting potential for nanocompsite as novel alternatives to cancer therapy. Our molecular data showed
that both mRNA and protein levels of tumor suppressor gene p53 were upregulated and induce activity
of DNA fragmentation in liver cells.
Keywords
curcumin nanoparticles; Zinc Oxide Nanoparticles; P53- gene expression
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