Possible Ameliorative Effect Of Platelet Rich Plasma On Testicular Toxicity Of Silver Nanoparticles In Adult Albino Rats | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 67, Issue 6, June 2024, Page 67-76 PDF (947.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2023.231340.8504 | ||||
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| Authors | ||||
Marwa El-Soudy Shakra1; Nihal Abdelmoneim Mahmoud 2; Samah Gouda Ahmed3; Safaa Abdelfattah Elsharkawy 4; Amina Mohamed Tolba5; Rehab Ali Masoud1; Sara Nagdy Mousa 2; Neveen Adel Ebrahim  6
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| 1Department of Forensic Medicine and Clinical Toxicology. Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||||
| 2Department of Physiology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||||
| 3Department of Histology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||||
| 4Department of Community Medicine and Occupational Health, Faculty of Medicine for Girls, Al-Azhar University,Cairo, Egypt. | ||||
| 5Department of Anatomy, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||||
| 6Department of Forensic Medicine and Clinical Toxicology. Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Abstract Silver nanoparticles (AgNPs) are being used increasingly in industrial and medical products owing to their unique properties. AgNPs can penetrate the testicular blood barrier and cause disorders in the male reproductive system. Platelet-rich plasma (PRP) contains many of growth factors that have regenerative and anti-inflammatory characteristics and have been used in different medical fields. In this work, we examined the toxicity of AgNPs and the possible ameliorative effect(s) of PRP on adult rat testes. Experimental study was conducted on 24 mature albino rat males that were allocated into four equal groups: G1: kept as control. G2: AgNPs-intoxicated group. G3: PRP–treated group. G4: recovery group. We collected samples for biochemical, immunohistochemical, histological, and genetic marker analysis. We found that AgNPs administration decreases glutathione (GSH), reproductive hormones, apoptotic marker; Bcl-2, germinal epithelial height, mRNA expression of claudin, and an increase in MDA level, TNF-α, lipid profile, thickening of the tubules' basement membrane with various histopathological defects, and an increase in mRNA expression of connexion-43. However, these negative effects were enhanced after PRP treatment. In conclusion, AgNPs changed the male reproductive system's biochemical and structural composition and administration of PRP effectively alleviating these changes. So, the scientific community ought to devote care to establishing procedures for eliminating toxicity, promoting health awareness, and reducing exposure to such materials. | ||||
| Keywords | ||||
| Silver nanoparticle; Platelet rich plasma; Testicular toxicity; ROS; Connexion-43; Bcl-2 | ||||
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