The Role of Interleukin-17 and Toll-Like Receptor 4 Gene Polymorphisms in Patients with Hepatitis C Virus and Hepatocellular Carcinoma | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 33, Issue 1, January 2024, Page 57-63 PDF (375.16 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2024.327018 | ||||
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| Authors | ||||
Doaa A. Shaker1; Ahmed S. Elgazar2; Rana A. Khashaba3; Ola S. El-Shimi   4; Shaymaa M. Abdelrahman5; Walaa M. Ibrahim6; Riham N. Ragab1
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| 1Microbiology and Immunology Department, Faculty of Medicine, Benha University, Benha, Egypt | ||||
| 2Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine, Benha University, Benha, Egypt | ||||
| 3Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt; Faculty of Medicine, King Salman International University (KSIU), El Tur Branch, Egypt | ||||
| 4Clinical and Chemical Pathology Department, Faculty of Medicine, Benha University, Benha, Egypt | ||||
| 5Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Benha, Egypt | ||||
| 6Internal Medicine Department, Faculty of Medicine, Benha University, Benha, Egypt | ||||
| Abstract | ||||
| Background: Hepatocellular carcinoma (HCC) is a global health problem. HCC is the fourth common malignancy in Egypt. It is a crucial medical need to predict hepatocellular carcinoma development in cirrhotic liver patients. Objectives: this study aims to investigate the association between TLR4 gene polymorphism (rs2149356) and the probability of HCC development among chronic hepatitis C virus infected patients and clarify the role of serum IL-17 in patients with end stage liver disease and HCC in HCV infected patients. Methodology: the study included 25 patients with chronic hepatitis C infection, 25 patients with chronic hepatitis C infection and HCC, and 25 apparently healthy control subjects as controls. All participants undergone full clinical and laboratory assessment. TLR4 (rs2149356) G/T polymorphism genotyping by PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism) and serum IL-17 detection by ELISA (enzyme-linked immunosorbent assay). Results: TLR4 (rs2149356) GG genotype was significantly associated with chronic HCV with HCC patients (72%) and chronic HCV patients (48%), while GT genotype was associated with healthy controls (48%) (P-value <0.001). Serum IL-17 was significantly elevated in chronic HCV with HCC patients than chronic HCV and control subjects (P-value <0.001). Serum IL-17 was an excellent predictor for HCC development at a cut-off value of 128.1 pg/dL with 96% sensitivity and 82% specificity. Conclusion: TLR4 (rs2149356) polymorphism and IL-17 have an important role in immunopathogenesis of HCC in chronic HCV infected patients. | ||||
| Keywords | ||||
| Hepatocellular carcinoma; Hepatitis C virus; TLR4; IL-17 | ||||
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