The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.
El Hennawi, D., Ahmed, M., El Tabbakh, M., Abdelrehem, A., Dessouki, A. (2024). The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.. EKB Journal Management System, 29(1), 1-8. doi: 10.21608/cat.2023.238196.1205
Diaa El-Dein Mohamed El Hennawi; Mohamed Rifaat Ahmed; Mohamed Tawfik El Tabbakh; Ahmed Salama Abdelrehem; Amina Ali Dessouki. "The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.". EKB Journal Management System, 29, 1, 2024, 1-8. doi: 10.21608/cat.2023.238196.1205
El Hennawi, D., Ahmed, M., El Tabbakh, M., Abdelrehem, A., Dessouki, A. (2024). 'The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.', EKB Journal Management System, 29(1), pp. 1-8. doi: 10.21608/cat.2023.238196.1205
El Hennawi, D., Ahmed, M., El Tabbakh, M., Abdelrehem, A., Dessouki, A. The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.. EKB Journal Management System, 2024; 29(1): 1-8. doi: 10.21608/cat.2023.238196.1205

The Effect of Sildenafil Citrate (Viagra®) on Nasal Olfactory Mucosa of Adult Male Albino Rat: caspase-3 and inducible nitric oxide synthase protein expressions.

Catrina: The International Journal of Environmental Sciences
Volume 29, Issue 1 - Serial Number 29, March 2024, Page 1-8  XML PDF (995.35 K)
Document Type: Original Article
DOI: 10.21608/cat.2023.238196.1205
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Authors
Diaa El-Dein Mohamed El Hennawi1; Mohamed Rifaat Ahmed2; Mohamed Tawfik El Tabbakh2; Ahmed Salama Abdelrehem2; Amina Ali Dessouki email orcid 3
1Otorhinolaryngology Department, Suez Canal University Faculty oF Medicine, Ismailia, Egypt
2Otorhinolaryngology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
3Pathology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Abstract
Like all human senses, the sense of olfaction (smell) plays an important role in person’s life. The sense of smell serves as a first warning signal, warning to the smoke of a fire, spoiled food, or the odor of a natural gas leak or dangerous fumes. Olfactory mucosa is a component of nervous system which regenerate throughout person's lifetime. Dysfunction of olfactory sensitivity may be a side effect of chronic use of high sildenafil doses. Sildenafil is oral drug effective for erectile dysfunction in men, and approximately 20% of patients complain about nasal congestion after sildenafil administration. Aim of this study was to evaluate effect of long-term sildenafil administration on rat olfactory mucosa. Forty-five male albino rats were divided into three equal groups, group I kept as control. Rats in groups II and III received sildenafil citrate orally at doses of 50 mg/kg and 100 mg/kg, respectively for three months. At the end, olfactory mucosa removed, fixed in proper fixations for histopathology, immunohistochemistry, and SEM. Results revealed that rats treated with high dose of sildenafil revealed loss of smell as well as alteration of histological pictures, immunohistochemical staining of apoptotic markers and SEM of olfactory mucosa while the rats in group II revealed normal sense of olfaction and normal histology of olfactory mucosa. In conclusion, chronic use of 100 mg sildenafil citrate led to olfactory dysfunction through stimulation of iNOS and Caspase 3 apoptotic markers whereas low dose needs further investigation and may introduce a prospective therapy for a post COVID 19 dysfunction.
Keywords
Caspase 3; Electron microscope; iNOS; Olfactory mucosa
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