THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS
Siraj, S., Ahmed, W., Abd El-Tawab, A., Elhofy, F., Elmasry, D. (2023). THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS. EKB Journal Management System, 46(2), 1169-1183. doi: 10.21608/bfsa.2023.327554
Suhair Sh. AL. Siraj; Wedad Ahmed; Ashraf A. Abd El-Tawab; Fatma I. Elhofy; Dalia M.A Elmasry. "THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS". EKB Journal Management System, 46, 2, 2023, 1169-1183. doi: 10.21608/bfsa.2023.327554
Siraj, S., Ahmed, W., Abd El-Tawab, A., Elhofy, F., Elmasry, D. (2023). 'THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS', EKB Journal Management System, 46(2), pp. 1169-1183. doi: 10.21608/bfsa.2023.327554
Siraj, S., Ahmed, W., Abd El-Tawab, A., Elhofy, F., Elmasry, D. THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS. EKB Journal Management System, 2023; 46(2): 1169-1183. doi: 10.21608/bfsa.2023.327554

THYME AND CUMIN NANOEMULSION AS A PROMISING ANTIMICROBIAL AGENT AGAINST MULTIDRUG-RESISTANT STAPHYLOCOCCUS AUREUS

Bulletin of Pharmaceutical Sciences Assiut University
Volume 46, Issue 2, December 2023, Page 1169-1183  XML PDF (1.13 MB)
Document Type: Original Article
DOI: 10.21608/bfsa.2023.327554
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Authors
Suhair Sh. AL. Siraj1; Wedad Ahmed email 2; Ashraf A. Abd El-Tawaborcid 3; Fatma I. Elhofy3; Dalia M.A Elmasry4
1Biology Department, Faculty of Science, Mustansiriyah University, Bagdad, Iraq
2Bacteriology,Mycology and Immunology department , Faculty of veterinary medicine, Benha university
3Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
4Nanomaterials Research and Synthesis Unit, Animal Health Research Institute, Agricultural Research Center (ARB), Giza, Egypt
Abstract
This study aimed to compare thyme, cumin extracts, and their nanoemulsion as antibacterial on S.aureus. The methods include , preparation of aqueous, oil extract and  nanoemulsion from thyme and cumin, determination of  MIC using the resazurin microdilution method, detection of mecA and coagulase genes by PCR before and after the treatment with nanoemulsions, figuring the bacteria after treatment using TEM. the result showed that the MIC values of thymus oil 100% , thymus oil nano emulsion 20% , cumin oil 100% ,cumin oil nanoemulsion 20% ,mix oil 20% ,thymus oil 20% ,cumin oil 20%  ( 0.048, 0.156 , 1.562 ,10 , 0.625 , 0.312 , 10 ,) mg/m  respectively , mecA and coagulase genes was absent after the in vitro treatment and TEM microscope showed the bacteria with extensive damage to the cell membrane. the result showed that nanoemulsion has a higher effect on S. aureus than  essential oil alone
Keywords
Thymus; cumin; nanoparticle; antibacterial activity; TEM microscope
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