THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS
Badr, G., El-Hossary, F., Salah, M., Khalaf, M., Sayed, E., Elminshawy, A. (2023). THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS. EKB Journal Management System, 46(2), 1285-1300. doi: 10.21608/bfsa.2023.327685
Gamal Badr; Fayez M. El-Hossary; Mohamed Salah; Mohamed Khalaf; Eman A. Sayed; Ahmed Elminshawy. "THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS". EKB Journal Management System, 46, 2, 2023, 1285-1300. doi: 10.21608/bfsa.2023.327685
Badr, G., El-Hossary, F., Salah, M., Khalaf, M., Sayed, E., Elminshawy, A. (2023). 'THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS', EKB Journal Management System, 46(2), pp. 1285-1300. doi: 10.21608/bfsa.2023.327685
Badr, G., El-Hossary, F., Salah, M., Khalaf, M., Sayed, E., Elminshawy, A. THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS. EKB Journal Management System, 2023; 46(2): 1285-1300. doi: 10.21608/bfsa.2023.327685

THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS

Bulletin of Pharmaceutical Sciences Assiut University
Volume 46, Issue 2, December 2023, Page 1285-1300  XML PDF (1.57 MB)
Document Type: Original Article
DOI: 10.21608/bfsa.2023.327685
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Authors
Gamal Badr email orcid 1; Fayez M. El-Hossary2; Mohamed Salah3; Mohamed Khalaforcid 2; Eman A. Sayed4; Ahmed Elminshawy5
1Zoology Department, Faculty of Science, Assiut University
2Physics Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt
3Botany and Microbiology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
4Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt
5Department of Cardiothoracic Surgery, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
 
Impaired healing process of diabetic wounds is a common problem among diabetic patients. These diabetic wounds are slow to heal and can lead to serious complications if left untreated. One of the reasons diabetic wounds are slow to heal is that pathogenic bacteria often colonize them. Cold atmospheric plasma (CAP) is a promising new technology that has been shown to effectively kill and inhibit pathogenic bacteria in diabetic wounds. In the current study, we examined by what means CAP works and its potential impact as a diabetic wound treatment. Fifty diabetic patients aged from 20 to 60 years old with diabetic wounds were included in our study using the swab method. We used general and specific culture media to isolate the pathogenic bacteria from diabetic wounds and identified the bacteria via different techniques using a light microscope (oil lens) and VITEK. Moreover, we confirmed the identification of isolated bacteria by 16S rRNA. Our results demonstrated the direct anti-bacterial effect of CAP against the isolated bacteria. The most prominent bacterial type in diabetic wounds was Staphylococcus sp. CAP demonstrated the highest effect against Bacillus sp. and Paenibacillus sp., which were responsible for relevant changes in the skin and were associated with the skin immune response and microbiota. Our results revealed the therapeutic use of CAP as a powerful strategy to overcome the invading pathogenic bacteria that colonize diabetic wounds and hence improve the healing process of diabetic wounds.
Keywords
Bacterial colonization; cold atmospheric plasma; diabetic wounds; Paenibacillus species, pathogenic bacteria
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