POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED
Hendrarti, W., Robiansyah, M., Nur, S., Amin, A., nisa, M., Abidin, H. (2023). POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED. EKB Journal Management System, 46(2), 1259-1268. doi: 10.21608/bfsa.2023.327712
Wahyu Hendrarti; Muhammad Robiansyah; Syamsu Nur; Astuti Amin; Michrun nisa; Hamdayani Lance Abidin. "POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED". EKB Journal Management System, 46, 2, 2023, 1259-1268. doi: 10.21608/bfsa.2023.327712
Hendrarti, W., Robiansyah, M., Nur, S., Amin, A., nisa, M., Abidin, H. (2023). 'POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED', EKB Journal Management System, 46(2), pp. 1259-1268. doi: 10.21608/bfsa.2023.327712
Hendrarti, W., Robiansyah, M., Nur, S., Amin, A., nisa, M., Abidin, H. POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED. EKB Journal Management System, 2023; 46(2): 1259-1268. doi: 10.21608/bfsa.2023.327712

POTENTIAL OF ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA L.) LEAVES AS ADJUVANT-ANTIBIOTIC ON SALMONELLA TYPHI CHARACTERIZED

Bulletin of Pharmaceutical Sciences Assiut University
Volume 46, Issue 2, December 2023, Page 1259-1268  XML PDF (853.03 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2023.327712
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Authors
Wahyu Hendrarti email 1; Muhammad Robiansyah1; Syamsu Nurorcid 2; Astuti Amin2; Michrun nisa3; Hamdayani Lance Abidin4
1Department Pharmacology and Clinical Pharmacy, Sekolah Tinggi Ilmu Farmasi Makassar, 90242, Makassar, Indonesia
2Depratment of Pharmaceutical Analysis and Medicinal Chemistry, Sekolah Tinggi Ilmu Farmasi Makassar, 90242, Makassar, Indonesia
3Depratment of Pharmaceutical Technology, Sekolah Tinggi Ilmu Farmasi Makassar, 90242, Makassar, Indonesia
4Depratment of Pharmaceutical Biology, Sekolah Tinggi Ilmu Farmasi Makassar, 90242, Makassar, Indonesia
Abstract
Guava (Psidium guajava L.) contains many chemical compounds, including quercetin, myricetin, epicatechin, and tannins which have been shown to have efficacy as an efflux pump inhibitor (EPI) in several pathogenic bacteria. This study aimed to investigate the synergistic effect of the ethanol extract of guava leaves with five common antibiotics on Salmonella typhi isolates expressing active efflux pumps. Bacterial characterization was carried out on clinical isolates of S. typhi, and molecular resistance mechanisms were determined using qRT-PCR by measuring the expression of the Acriflavine resistance protein B (AcrB) efflux pump. Guava leaves were extracted by maceration using 70% ethanol. Tests were carried out using the diffusion method with tetracycline, ampicillin, trimethoprim-sulfamethoxazole, chloramphenicol, and ciprofloxacin. The extract was made in serial concentrations of 0.01, 0.025, 0.05, 0.075, and 0.1%. The results showed that the tested isolates decreased the expression of AcrB from 11.48167 to 7.38818 ρg/mL after adding CCCP. The synergistic effect occurred at (1) extract concentration of 0.1% against tetracycline 30 μg, (2) concentration of 0.05% against ampicillin10μg, (3) concentration of 0.075% against trimethoprim-sulfamethoxazole 23.75-1.25 μg, (4) concentration of 0.05% against chloramphenicol 30 μg, (5) concentration of 0.025% against ciprofloxacin 5 µg. The ethanol extract of guava leaves (Psidium guajava L.) synergized with the studied antibiotics and displayed efflux pump inhibitory activity. Consequently, it could serve as a natural antibiotic adjuvant candidate.
Keywords
Antibiotics; efflux pump inhibitor; guava leaves; Salmonella thypi ST019
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