Potential prophylactic effect of Naringenin loaded liposome eye drops on streptozotocin-induced diabetic cataract in rats | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 67, Issue 6, June 2024, Page 49-59 PDF (1.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2023.226874.8431 | ||||
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| Authors | ||||
Amany Abdel-Ghaffar1; Medhat W. Shafaa2; Hossam S. Kareem3; Marwa A. Fouly4; Hadeer Rashad5; Amany M. Shaban  5
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| 1Biochemistry Department, Research Institute of Ophthalmology, Giza, Egypt. | ||||
| 2Physics Department, Faculty of Science, Helwan University, Cairo, Egypt. | ||||
| 3Physiological Optic Unit, Vision Science Department, Research Institute of Ophthalmology, Giza, Egypt. | ||||
| 4Medical Retina Department, Research Institute of Ophthalmology, Giza, Egypt. | ||||
| 5Chemistry Department, Biochemistry division, Faculty of Science, El Fayoum University, El Fayoum, Egypt. | ||||
| Abstract | ||||
| Diabetes may lead to oxidative stress-related ocular complications including cataract. Thus, the aid of antioxidant therapy versus oxidative stress development is necessary to reduce cataract progression. Flavonoid Naringenin is a well- recognized natural antioxidant. Liposomes are appropriate nano-carriers for ocular drug delivery. Hence, we aimed to prepare and assess the potential of Naringenin loaded liposome (NLL) as topical ocular drug to ameliorate streptozotocin (STZ)-induced diabetic cataract. NLL was formulated by thin lipid film method (0.241 mg/ml); its characterization was confirmed by Transmission Electron Microscope (TEM) imaging, zeta potential and Differential Scanning Calorimetry (DSC) measurements. 60 male Wistar rats have been distributed into three groups (control, (STZ) - induced diabetic cataract and Naringenin treated). NLL was administered as eye drops (in treated group) twice a day for 12 weeks. Slit-lamp photography and lens inspection were conducted, FBS was detected. In the lens tissue reduced glutathione (GSH), total soluble lens protein (TSP), carbonyl protein (CP), interleukin- 6 (IL-6), Caspase-3 and vascular endothelial growth factor (VEGF) were measured. TEM imaging, particle size, zeta potential and DSC results exhibited an effective NLL preparation. NLL exhibited antioxidant, anti-inflammatory and antiapoptotic efficacy in all parameters associated with oxidative stress in the lens tissue of type 1 diabetic rats. Finally, the findings disclosed that NLL could be recommended as a potential prophylactic agent in diabetic cataract. | ||||
| Keywords | ||||
| Naringenin; Liposomes; DSC; Diabetes; Cataract; Oxidative stress | ||||
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