Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis
Aboutaleb, A., Hegazy, E., Elshimy, A., Amer, M., Kotb, M. (2024). Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis. EKB Journal Management System, 33(1), 91-98. doi: 10.21608/ejmm.2024.328335
Alaa M. Aboutaleb; Essraa A. Hegazy; Amal A. Elshimy; Marwa A. Amer; Maha M. Kotb. "Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis". EKB Journal Management System, 33, 1, 2024, 91-98. doi: 10.21608/ejmm.2024.328335
Aboutaleb, A., Hegazy, E., Elshimy, A., Amer, M., Kotb, M. (2024). 'Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis', EKB Journal Management System, 33(1), pp. 91-98. doi: 10.21608/ejmm.2024.328335
Aboutaleb, A., Hegazy, E., Elshimy, A., Amer, M., Kotb, M. Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis. EKB Journal Management System, 2024; 33(1): 91-98. doi: 10.21608/ejmm.2024.328335

Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis

Egyptian Journal of Medical Microbiology
Volume 33, Issue 1, January 2024, Page 91-98  XML PDF (387.95 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2024.328335
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Authors
Alaa M. Aboutaleb email 1; Essraa A. Hegazy1; Amal A. Elshimy1; Marwa A. Amer2; Maha M. Kotb1
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
2Department of Dermatology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
Abstract
Background: Psoriasis is a persistent, immune-related complex condition that predominantly impacts both the skin and joints, involving multiple factors. Objectives: Our study aimed to make a comparison between the PBMCs expression levels of NLRP3 and plasma IL-21 levels in psoriatic patients with that of healthy controls and a correlation between these levels and the severity of psoriasis. Methodology: This observational cross-sectional study was performed on 40 psoriatic patients and 40 healthy individuals who served as the control group. We assessed the expression levels of NLRP3 in PBMCs using real-time reverse transcription-polymerase chain reaction (RT-PCR), while plasma levels of IL-21 were determined using the enzyme-linked immunosorbent (ELISA) technique. Results: Psoriatic patients showed significantly higher PBMCs expression levels of NLRP3 (P< 0.001), and insignificantly different plasma IL-21 levels from those in the control group. The expression levels of NLRP3 demonstrated a significant positive correlation with the severity of psoriasis (P< 0.001). In contrast, there was no significant correlation detected between IL-21 levels and the severity of psoriasis. Additionally, no significant correlation was observed between NLRP3 and IL-21 levels. Conclusions: NLRP3 could be a potential diagnostic and prognostic biomarker in psoriasis diagnosis with 92.5% sensitivity and 90% specificity and a promising therapeutic target. Additional studies are warranted to examine the exact contribution of IL-21 to the pathogenesis of psoriasis.
Keywords
Inflammasome NLRP3; Plasma Interleukin-21; Psoriasis; PBMCs
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