Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves
Yousif, A., Abdel-Khalek, A., elsherbeny, M., Elhayes, A., GAbr, S., Mahmoud, R. (2023). Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves. EKB Journal Management System, 2(4), 81-90. doi: 10.21608/jsaes.2023.246737.1061
Ahmed Ibrahim Yousif; Abdel-Khalek El-Sayed Abdel-Khalek; mohamed Abd El Gawad elsherbeny; Abdelaziz Elhayes; sherif A GAbr; Raed Mahmoud. "Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves". EKB Journal Management System, 2, 4, 2023, 81-90. doi: 10.21608/jsaes.2023.246737.1061
Yousif, A., Abdel-Khalek, A., elsherbeny, M., Elhayes, A., GAbr, S., Mahmoud, R. (2023). 'Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves', EKB Journal Management System, 2(4), pp. 81-90. doi: 10.21608/jsaes.2023.246737.1061
Yousif, A., Abdel-Khalek, A., elsherbeny, M., Elhayes, A., GAbr, S., Mahmoud, R. Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves. EKB Journal Management System, 2023; 2(4): 81-90. doi: 10.21608/jsaes.2023.246737.1061

Visual Examination and Computer Assessed Sperm Analysis (Casa) Of Goat Semen Diluted by Tris-Extender Supplemented with Aqueous Extract of Moringa Oleifera Leaves

Journal of Sustainable Agricultural and Environmental Sciences
Volume 2, Issue 4, November 2023, Page 81-90  XML PDF (381.48 K)
Document Type: Original research paper
DOI: 10.21608/jsaes.2023.246737.1061
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Authors
Ahmed Ibrahim Yousif email orcid 1; Abdel-Khalek El-Sayed Abdel-Khalek2; mohamed Abd El Gawad elsherbeny3; Abdelaziz Elhayes4; sherif A GAbr5; Raed Mahmoud4
1Animal Production Research Institute (APRI), Agricultural Research Center (ARC).
2Animal Production Department, Mansoura University, Mansoura, Egypt
3Animal Production Research Institute, Agricultural Research Center, Egypt.
4Animal Production Department, Faculty of Agriculture, Tanta University, Egypt.
51Department of Animal Production, Faculty of Agriculture, Tanta University, Egypt
Abstract
The aim of the current study was to assess the impacts of aqueous extract of Moringa oleifera leaves (MOLE) on semen quality and sperm freezing ability of bucks. 5 sexually mature Damascus bucks (65-75 kg live body weight, 2-4 years of age) were used for collecting semen once per week for seven weeks. Only semen with a mass motility of ≥ 70% was pooled. Pooled semen had been divided into 4 aliquots, with the free extender (C=control, no MOLE) and the extenders supplemented with MOLE at levels of (10, 20, and 30 µl/mL) as an antioxidant. Tris-citric acid-extender was used to dilute semen at a rate of 1:20 using different MOLE levels, then chilled to 5 °C for two hours (equilibration period), placed into 0.25 mL straws and finally kept in liquid nitrogen at -196 oC for two weeks. After dilution, equilibration, and thawing, semen was assessed visually and via CASA. The results showed that MOLE, at a level of 30 µl/ml, had a beneficial effect on all sperm characteristics, all types of sperm motility, and normal forms assisted by CASA in post-diluted semen, after equilibration and thawing. The highest MOLE level (30 µl/ml) considerably improved the sperm kinetic indexes and all sperm velocity types (VCL, VSL, and VAP).
In conclusion, Moringa oleifera leaves (30 µl) in semen extender of bucks is good tool for improving semen quality and sperm freezing ability of bucks
Keywords
Cryopreservation; CASA; Damascus; Tris; semen
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