Impact of TLR9 gene Polymorphism on Severity of Helicobacter pylori Infection and Its Association with Virulence genes | ||||
Egyptian Journal of Medical Microbiology | ||||
Volume 33, Issue 1, January 2024, Page 99-110 PDF (673.59 K) | ||||
Document Type: New and original researches in the field of Microbiology. | ||||
DOI: 10.21608/ejmm.2024.328759 | ||||
View on SCiNiTO | ||||
Authors | ||||
Yara E. Marei1; Bassam Mansour2; Samar A. Ahmed3; Hassnaa M. Nassar4; Eman F. Nasr El Dien5; Asmaa B. Hamady 1 | ||||
1Medical Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
2Infection and endemic disease Department, Faculty of medicine, Suez Canal University, Ismailia, Egypt | ||||
3Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
4Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
5Internal Medicine Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt | ||||
Abstract | ||||
Background: Helicobacter pylori colonizes stomach epithelium in the majority of people worldwide. It disturbs the local mucosa's homeostasis in the stomach and causes many pathological disorders, such as chronic gastritis, peptic ulcers and gastric cancer. Objective: This study aimed to determine the relation between different TLR9 gene polymorphism genotypes and the risk and severity of H. pylori‑related gastric diseases and determine possible associations of these genotypes with H. pylori virulence genes. Methodology: 136 adult dyspeptic patients who had gastric symptoms attended Gastroenterology Department at Suez Canal University Hospitals were incorporated in this study. Three stomach biopsies were obtained from each patient. The glmM gene was amplified using PCR for confirmation of H. pylori infection. Virulence genes were identified by PCR. Using PCR-RFLP, the genotypes of TLR9 gene polymorphism were analyzed. Results: The most prevalent genes were hsp60, vacA s1/ s2 and vacA m1/m2 detected in 93.7 % of H. pylori isolates, followed by cagA (54.2%). Frequencies of CC, TC, and TT genotypes were 41.7 %, 38.5 %, and 19.8 %, respectively in infected patients compared to 10.4 %, 54.2 %, and 35.4 %, respectively in the control group. Conclusion: This study reported a significant association between TLR9 SNP and H. pylori‑related diseases and showed that the patients who had the CC genotype may have higher risk of developing severe gastritis. There may be a synergistic effect between the CC genotype and H. pylori carrying hsp60 and vacA s1/m1 genes leading to severe gastritis. | ||||
Keywords | ||||
H. pylori; PCR; TLR9 gene polymorphism; virulence genes | ||||
Statistics Article View: 181 PDF Download: 71 |
||||