Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties
Oladejo, B., Olalemi, A., Oyekanmi, O., Tabiti, S. (2023). Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties. EKB Journal Management System, (), -. doi: 10.21608/mid.2023.227033.1579
Babayemi Olawale Oladejo; Adewale Oluwasogo Olalemi; Oloyede Joshua Oyekanmi; Success Peter Tabiti. "Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties". EKB Journal Management System, , , 2023, -. doi: 10.21608/mid.2023.227033.1579
Oladejo, B., Olalemi, A., Oyekanmi, O., Tabiti, S. (2023). 'Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties', EKB Journal Management System, (), pp. -. doi: 10.21608/mid.2023.227033.1579
Oladejo, B., Olalemi, A., Oyekanmi, O., Tabiti, S. Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties. EKB Journal Management System, 2023; (): -. doi: 10.21608/mid.2023.227033.1579

Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties

Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 08 December 2023  XML PDF (811.5 K)
Document Type: Original Article
DOI: 10.21608/mid.2023.227033.1579
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Authors
Babayemi Olawale Oladejo email orcid 1; Adewale Oluwasogo Olalemi1; Oloyede Joshua Oyekanmi2; Success Peter Tabiti1
1Department of Microbiology, Federal University of Technology, Akure, Nigeria.
2Inqaba Biotech West Africa, Moniya 200136, Ibadan, Nigeria
Abstract
Background:  Water quality monitoring is useful for promoting sustainable development of water resources. Objective: The aim of this study was to determine adenovirus concentrations in water samples from River Owena, Nigeria. Methods: The physicochemical characteristics of the water samples were determined immediately after collection using standard methods. A quantitative SYBR green-based real-time PCR assay for detecting human adenovirus-F (HAdV-F) was optimized using DNA extracted with a ZymoBIOMICS™ DNA Miniprep kit from filtered water samples. Results: Findings revealed that HAdV-F genes with end relative fluorescence units (RFUs) ranged from 7206.31 to 14248.55 and corresponding quantification cycles (Cq) ranged from 13.32 to 18.38, while the non-template control (NTC) was 31.71 in a 44-cycle reaction. Water temperature ranged from 34 to 36℃ and pH 6.4 to 7.4, with total solids between 945 and 994 mg/l. Conclusion: Adequate treatment of the water from the river must be a priority by residents who rely on the water for domestic activities.
Keywords
Adenovirus; Physicochemical; Water quality; SYBR qPCR
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