Isolation and Transformation Mi gene Using Chitosan Nanoparticle Into Cucumber to Resist Root-Knot Nematode | ||||
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
Volume 15, Issue 2, December 2023, Page 813-824 PDF (677.65 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsc.2023.329312 | ||||
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Authors | ||||
Amira H. Ayad1; Ashraf B. Abdel Razik1; Eman T. Hussein2; Entesar H. Taha3; Samir A. Ibrahim1; Sawsan Y. Elateek1 | ||||
1Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt. | ||||
2Department of Botany and Microbiology, Faculty of Science, Helwan University, Egypt. | ||||
3Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Egypt. | ||||
Abstract | ||||
Many plants around the world suffer significantly from plant parasitic nematodes, so transgenic crops were suggested as an application of biotechnology to make plants more resistant to nematodes in a safe way and without causing any changes in the ecosystem of plants. There are a few strategies for controlling nematodes in cucumber, and one of these methods uses natural resistance genes (R-genes) to resist nematodes. Our study was conducted with an isolated mi gene from nematode-resistant tomato plants, which produce proteins capable of preventing nematode feeding and detected with the PCR method then the resistant gene was inserted into a pEGAD vector and carried out in chitosan nanoparticles to be transformed into plants (cucumber cultivars) using Injections. The transgenic plants were tested by using spore suspension on a leaf. As a result of this study, a UV/visible spectroscope was used to characterise chitosan nanoparticles, and GFP gene florescent light found in transgenic plants compared to control. This will be a new approach to controlling nematodes, not only with genetic engineering but also with chitosan nanoparticles to get new cucumber cultivars to resist nematodes. | ||||
Keywords | ||||
Plant-parasitic Nematodes; Root-Knot Nematode (Meloidogyne); Chitosan Nanoparticles; Mi gene; RT-PCR | ||||
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