Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt
Eissa, S., Abdelaziz, E., Hassan, A., Mohamed, Y., Ouda Ahmed, S., Elshabiny, L. (2024). Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt. EKB Journal Management System, 55(3), 851-861. doi: 10.21608/ejvs.2023.245264.1658
Sabry Ismail Eissa; Eid El saeed Abdelaziz; Abdelwahed Mahmuod Hassan; Yousreya Hashim Mohamed; Sahar El-Sayed Ouda Ahmed; laila Mostafa Elshabiny. "Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt". EKB Journal Management System, 55, 3, 2024, 851-861. doi: 10.21608/ejvs.2023.245264.1658
Eissa, S., Abdelaziz, E., Hassan, A., Mohamed, Y., Ouda Ahmed, S., Elshabiny, L. (2024). 'Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt', EKB Journal Management System, 55(3), pp. 851-861. doi: 10.21608/ejvs.2023.245264.1658
Eissa, S., Abdelaziz, E., Hassan, A., Mohamed, Y., Ouda Ahmed, S., Elshabiny, L. Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt. EKB Journal Management System, 2024; 55(3): 851-861. doi: 10.21608/ejvs.2023.245264.1658

Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt

Egyptian Journal of Veterinary Sciences
Volume 55, Issue 3, May and June 2024, Page 851-861  XML PDF (1 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2023.245264.1658
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Authors
Sabry Ismail Eissa1; Eid El saeed Abdelaziz2; Abdelwahed Mahmuod Hassan3; Yousreya Hashim Mohamed4; Sahar El-Sayed Ouda Ahmed email orcid 5; laila Mostafa Elshabiny6
1Mycoplasma department AHRI,ARC,GIZA,Egypt
2Mycoplasma Department ,AHRI,ARC,GIZA,EGYPT.
3Mycoplasma Department AHRI,ARC,GIZA,EGYPT.
4Mycoplasma Department AHRI,ARC,GIZA.EGYPT
5Department of mycoplasma AHRI, ARC, Giza Egypt
6Mycoplasma Department AHRI,ARC,Giza,EGYPT
Abstract
Background: 
Hemotropic mycoplasmas (HM) the non-cultivable bacteria cause infectious anemia in many animals and humans.
Objective: Molecular detection, partial sequencing and phylogenetic analysis of the 16S rRNA of haemoplasmas in some animal species in Egypt.
Method: Blood samples with anticoagulant were collected from jugular vein of camels, sheep, goats, dogs and cats. DNA was extracted and different PCR assays were applied and sequenced.
Results: Candidatus M. haemoovis was detected in 10% of sheep in Beni- Swef governorate and in 14.46% of goats in Gharbia and the PCR resulted in the specific fragment at 506 bp. Samples from both sheep and goats showed high similarity with the homologous species on GenBank. Candidatus Mycoplasma haemolamae of camels CMhl, was detected in Shalateen in 8.33% of examined camels andshowed a specific fragment at 310pb. Haemoplasma incidence in cats was 32% in Giza governorate. Candidatus M haemominutum (CMhm) and Candidatus M. turicensis (CMt) were detected in 16% of the examined cats and gave a specific fragments (202 bp)  and (138 bp) respectively. Mycoplasma haemofelis could not be detected at all. Candidatus M. haemominutum (CMhm) showed high similarity with the homologous species on GenBank. Candidatus Mycoplasma haematoparvum (CMhp( was detected in dogs in Dkahlia governorate with 22% incidence and gave specific band at 380bp.
Conclusion: Haemoplasmas are circulating in different animal species in Egypt and PCR technique is the accurate method for detection of haemoplasma and sequencing is confirmatory to the results. 
Keywords
Haemoovis; CMhl; CMhm; CMhp; PCR; Egypt
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