Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing
Hassan, S., Abdel-Salam, M., hanfy, A., Abd-Elhalim, H., Ahmed, N., Metry, E. (2023). Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing. EKB Journal Management System, 31(2), 157-171. doi: 10.21608/ajs.2023.198877.1517
Sally Mohammed Hassan; Mohamed Abdel-Salam; ayman hanfy; Haytham Mohamed Abd-Elhalim; Nouh Ahmed; Emad Anis Metry. "Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing". EKB Journal Management System, 31, 2, 2023, 157-171. doi: 10.21608/ajs.2023.198877.1517
Hassan, S., Abdel-Salam, M., hanfy, A., Abd-Elhalim, H., Ahmed, N., Metry, E. (2023). 'Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing', EKB Journal Management System, 31(2), pp. 157-171. doi: 10.21608/ajs.2023.198877.1517
Hassan, S., Abdel-Salam, M., hanfy, A., Abd-Elhalim, H., Ahmed, N., Metry, E. Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing. EKB Journal Management System, 2023; 31(2): 157-171. doi: 10.21608/ajs.2023.198877.1517

Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing

Arab Universities Journal of Agricultural Sciences
Volume 31, Issue 2, December 2023, Page 157-171  XML PDF (1.24 MB)
Document Type: Original Article
DOI: 10.21608/ajs.2023.198877.1517
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Authors
Sally Mohammed Hassan email orcid 1; Mohamed Abdel-Salamorcid 2; ayman hanfy2; Haytham Mohamed Abd-Elhalim3; Nouh Ahmed4; Emad Anis Metry1
1Department of Plant Genetic Transformation, Agricultural Genetic Engineering Research Institute - Agricultural Research Center, Giza, Egypt.
2Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
3School of Biotechnology, Badr University in Cairo, Badr City, 11829, Cairo, Egypt
4Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
Abstract
Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers
Keywords
CRISPR; Cold storage; Cold-induced sweetening (CIS); Genome editing; Monosaccharides; Starch phosphorylase
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