Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities
El-Shora, H., Ibrahim, M., Alfakharany, M. (2024). Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities. EKB Journal Management System, 5(3), 309-319. doi: 10.21608/ajbas.2023.248584.1197
Hamed M El-Shora; Mohsen E Ibrahim; Mohammad W. Alfakharany. "Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities". EKB Journal Management System, 5, 3, 2024, 309-319. doi: 10.21608/ajbas.2023.248584.1197
El-Shora, H., Ibrahim, M., Alfakharany, M. (2024). 'Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities', EKB Journal Management System, 5(3), pp. 309-319. doi: 10.21608/ajbas.2023.248584.1197
El-Shora, H., Ibrahim, M., Alfakharany, M. Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities. EKB Journal Management System, 2024; 5(3): 309-319. doi: 10.21608/ajbas.2023.248584.1197

Potential inhibition of selected plant extracts on tyrosinase activity from Aspergillus nidulans and their antioxidant activities

Alfarama Journal of Basic & Applied Sciences
Volume 5, Issue 3, July 2024, Page 309-319  XML PDF (936.47 K)
Document Type: Original Article
DOI: 10.21608/ajbas.2023.248584.1197
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Authors
Hamed M El-Shora email 1; Mohsen E Ibrahim2; Mohammad W. Alfakharany2
1Botany department, Faculty of Science, Mansoura University
2Botany Department, Faculty of Science, Port Said University
Abstract
Tyrosinase (EC 1.14.18.1) was obtained from Aspergillus nidulans (Eidam) G. Winter (AUMC No. 7147). It is an important enzyme in biosynthesis of melanin and hydroxylation L-tyrosine to dihydroxy-phenylalanine and oxidation to dopaquinone. Tyrosinase was purified from Aspergillus nidulans by ammonium sulphate precipitation, DEAE-cellulose and Sephadex G-200 with specific activity of 230.76 units mg/ protein and the molecular weight was 40 kDa. Ethanolic extracts from Morus alba fruits and leaves of Chenopodium album, Rumex dentatus, Eruca sativa and Urtica urens showed an inhibitory effect against tyrosinase activity. The extract from Rumex dentatus leaves expressed the lowest IC50 value (The half-maximal inhibitory concentration) (33.8%), while Urtica urens leaves expressed the highest IC50 value for tyrosinase inhibition (82.6 µg/ mL). The contents of total phenols and flavonoids are investigated in the various plant extracts. Morus alba fruit extract expressed the highest content of phenols (328 mg/ g) and flavonoids (241 mg/ g) compared to the other plant extracts. All plant extracts exhibited appreciable scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) method. Eruca sativa leaves exhibited the highest scavenging activity of 150.9 μg/ mL with DPPH and 142.4 μg/ mL with ABTS+ .This present article reveals that the potential of leaf extract from Eruca sativa is a hopeful whitening agent of natural source versus skin hyperpigmentation.
Keywords
Antioxidant; Aspergillus nidulans; inhibition; plant extracts; tyrosinase
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