Strelitzia reginae Leaves: Phytochemical Elucidation and Antimicrobial Assessment on Clinical Isolates and Standard Strains | ||||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||||
| Article 5, Volume 4, Issue 1, January 2024, Page 52-61 PDF (759.96 K) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/aijpms.2023.178758.1181 | ||||
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| Authors | ||||
Asmaa A. Mahmoud   1; Marwa S. Abu Bakr 1; Hatem S. Abbass 2, 3; Shaimaa M. Azzam 4; Nagwan Galal El Menofy 5; Abd El‑Salam I. Mohammed 2
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| 1Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo , Egypt | ||||
| 2Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University (Boys), Cairo11884 , Egypt. | ||||
| 3Department of Pharmacognosy, Faculty of Pharmacy, Sinai University - Kantara Branch, Ismailia 41636, Egypt. | ||||
| 4Department of Biochemistry, Egyptian Drug Authority (EDA), Formerly National Organization for Drug Control and Research (NODCAR), Giza, Egypt. | ||||
| 5Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo, Egypt. | ||||
| Abstract | ||||
| Strelitzia reginae is used in some African countries as a traditional medicine with very few phytochemical and biological assessments. The study aims to isolate and structure elucidate of S. reginae phytoconstituents using different chromatographic and spectral techniques. In addition to, antimicrobial assessment of ethyl acetate and n-butanol fractions on both clinical isolates and standard strains. Strelitzia reginae belongs to the family Strelitziaceae which is a rich source of biologically active phytoconstituents and has diverse therapeutic values. Strelitzia reginae leaves were fractionated into ethyl acetate and n-butanol, and five compounds were detected. Compounds were identified as kaempferol (1), quercetin (2), daucosterol (3), vitexin (4), and rutin (5). The isolated compounds were elucidated by their NMR and ESI-MS spectral data. Antimicrobial activity of Strelitzia reginae ethyl acetate and n-butanol fractions screened against six different bacterial strains (three Gram-positive, three Gram-negative, and one fungus isolate). Both fractions showed antimicrobial activities against Klebsiella pneumonia and Staphylococcus aureus which is methicillinresistant (MRSA) strains. As well, the activity of antimicrobial was detected against Proteus mirabilis clinical isolate from urine and MRSA. On the other hand, both fractions showed inadequate antimicrobial activities against the Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans clinical isolates compared to reference drugs. The antimicrobial results were in accordance with previously reported folk uses and attributed to the isolated phytoconstituents. | ||||
| Keywords | ||||
| Antimicrobial; clinical isolates; flavonoids; MRSA; sterols; Strelitzia reginae | ||||
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