Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach
Mohamed, S., Abdel-Latif, S., El-Ansary, A., Salim, A. (2024). Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach. EKB Journal Management System, 67(7), 513-524. doi: 10.21608/ejchem.2023.232340.8515
Sabrein Mohamed; Samir A. Abdel-Latif; Aida L. El-Ansary; Alyaa I Salim. "Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach". EKB Journal Management System, 67, 7, 2024, 513-524. doi: 10.21608/ejchem.2023.232340.8515
Mohamed, S., Abdel-Latif, S., El-Ansary, A., Salim, A. (2024). 'Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach', EKB Journal Management System, 67(7), pp. 513-524. doi: 10.21608/ejchem.2023.232340.8515
Mohamed, S., Abdel-Latif, S., El-Ansary, A., Salim, A. Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach. EKB Journal Management System, 2024; 67(7): 513-524. doi: 10.21608/ejchem.2023.232340.8515

Green Spectrophotometric Assessment for Molnupiravir Quantification in Pharmaceutical Formulations and Biological Fluids: Study of Structural Elucidation and Theoretical Approach

Egyptian Journal of Chemistry
Volume 67, Issue 7, July 2024, Page 513-524  XML PDF (2.41 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2023.232340.8515
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Authors
Sabrein Mohamed email 1; Samir A. Abdel-Latif2; Aida L. El-Ansary3; Alyaa I Salimorcid 4
11-Department of chemistry, Faculty of science, Cairo University, Giza, Egypt 2-Chemistry Department, College of Science, Jouf University, P.O. Box 2014, Sakaka, Saudi Arabia
2Chemistry Department, Faculty of Science, Helwan University
3Dept. of chemistry, Faculty of Science, Cairo University
4Basic science Department, Faculty of engineering and applied science, Nile University, Giza, Egypt
Abstract
The FDA-approved antiviral drug molnupiravir (MPV) was investigated using a green spectrophotometric approach. With the help of mass spectrometry, UV-visible spectroscopy, and Infrared Fourier transform spectroscopy, this drug was characterized and elucidated structurally. This approach depends on measuring the absorbance in doubly distilled water as a solvent at 240 and 285 nm. Variable parameters were estimated and optimized. Beer's law is up to 35.0 µg/mL under optimal conditions with a correlation coefficient (r2)>0.99. Based on this evaluation, the new method was found to be highly sensitive with a LOD and LOQ of 0.34-0.78 and 1.13-2.59 µg/mL, respectively. Analyses provided reproducible results with a 2.0% relative standard deviation (RSD). As a result of the application of the proposed method, MPV levels were successfully achieved in raw materials, pharmaceutical formulations, and biological fluids. The geometry and energy quantum mechanical calculations were also performed using Becke's three-parameter exchange functional method. Using water as a solvent, Lee-Yang-Parr correlation functional analysis (B3LYP/DFT) confirmed the measured results.
Keywords
Molnupiravir; Spectrophotometry; Molecular geometry; Covid
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