Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt
Nabil, R., Abdelkhalek, A., Behiry, S., Younes, H. (2023). Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt. EKB Journal Management System, 14(2), 77-83. doi: 10.21608/eajbsh.2023.331127
Rokaia Nabil; Ahmed Abdelkhalek; Said Behiry; Hosny Younes. "Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt". EKB Journal Management System, 14, 2, 2023, 77-83. doi: 10.21608/eajbsh.2023.331127
Nabil, R., Abdelkhalek, A., Behiry, S., Younes, H. (2023). 'Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt', EKB Journal Management System, 14(2), pp. 77-83. doi: 10.21608/eajbsh.2023.331127
Nabil, R., Abdelkhalek, A., Behiry, S., Younes, H. Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt. EKB Journal Management System, 2023; 14(2): 77-83. doi: 10.21608/eajbsh.2023.331127

Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt

Egyptian Academic Journal of Biological Sciences, H. Botany
Volume 14, Issue 2, December 2023, Page 77-83  XML PDF (657.85 K)
Document Type: Original Article
DOI: 10.21608/eajbsh.2023.331127
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Authors
Rokaia Nabil1; Ahmed Abdelkhalek2; Said Behiryorcid 1; Hosny Younes1
1Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
2Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt.
Abstract
Background: The Zucchini yellow mosaic virus (ZYMV) is well recognized as a very significant viral pathogen that causes substantial yield reductions in cucurbitaceous crops on a global scale. Methods: To detect and identify ZYMV in squash plants we used the serology reaction, transmission electron microscope, and molecular methods.  Results:  ZYMV was first isolated from naturally infected squash plants in the Alexandria governorate of Egypt, where it caused mosaic, yellowing, and deformation of the leaves. In addition to a molecular diagnosis utilizing RT-PCR, the identification relied on serological responses with antisera to CMV, SqMV, and ZYMV. Using centrifugation and PEG, the virus was cleaned. The A260/280 and A280/260 ratios of the purified virus were 1.38 and 0.72, respectively, which are consistent with those of a nucleoprotein.  2.36 mg of pure virus was recovered from per 100g of infected leaf material. The virus particles, as seen under an electron microscope after a partial purification preparation technique, have a flexuous filamentous structure and measure around 750 nm in length and 12 nm in diameter. Finally, the molecular investigation established the identification of the ZYMV isolate after a specific antiserum with a titer of 1:2.56×104 was generated and tested at the third week.   
Keywords
ZYMV; RT-PCR; ELISA; electron microscopy; Purification
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