Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.
Abdelmoteleb, M., Mohamed, A., Genidy, N., Abdel-Haleem, D. (2023). Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.. EKB Journal Management System, 16(4), 135-150. doi: 10.21608/eajbsa.2023.331271
Mohamed N. Abdelmoteleb; Amany Z. Mohamed; Noha A. Genidy; Doaa R. Abdel-Haleem. "Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.". EKB Journal Management System, 16, 4, 2023, 135-150. doi: 10.21608/eajbsa.2023.331271
Abdelmoteleb, M., Mohamed, A., Genidy, N., Abdel-Haleem, D. (2023). 'Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.', EKB Journal Management System, 16(4), pp. 135-150. doi: 10.21608/eajbsa.2023.331271
Abdelmoteleb, M., Mohamed, A., Genidy, N., Abdel-Haleem, D. Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.. EKB Journal Management System, 2023; 16(4): 135-150. doi: 10.21608/eajbsa.2023.331271

Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.

Egyptian Academic Journal of Biological Sciences. A, Entomology
Volume 16, Issue 4, December 2023, Page 135-150  XML PDF (867.91 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2023.331271
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Authors
Mohamed N. Abdelmoteleb; Amany Z. Mohamed; Noha A. Genidy; Doaa R. Abdel-Haleemorcid
Entomology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Abstract
Neonicotinoids, which have a wide range of applications, are systemic insecticides that efficiently control several sucking pests. Recently, thiamethoxam (THIA) has become one of the popular pest control agents. In vivo study, the toxicity of THIA was evaluated against a laboratory strain of cowpea aphid (Aphis craccivora Koch.) using the leaf dipping method for 24 and 48 hrs of exposure. In silico study, THIA was docked to acetylcholine binding protein (AChBP) to explain their binding mode of interaction. THIA exhibited good toxicity against adult aphids with LC50 of 2.44 ppm at 24 hrs post-treatment, which greatly decreased to 0.75 ppm at 48 hrs post-treatment. THIA showed better binding interaction with AChBP than the co-crystalized ligand (imidacloprid) and good alignment in the active site. THIA indicated different variations in the activities of tested enzymes. Where, aphids treated with LC50 of THIA increased the biochemical activities of acetylcholinesterase, antioxidant enzymes and peroxidases while decreasing the activity of alpha esterase, carboxylesterase (CarE), glutathione S-transferase and catalase. Therefore, this compound is efficient against sap-sucking pests with good irreversible and specific binding interaction with nicotinic acetylcholine receptor (nAChR) and, consequently, low mammalian toxicity. So, THIA is considered a favourable agent for the management of insect pests.
Keywords
Aphis craccivora Koch; Thiamethoxam; Molecular Docking; nAChR; Biochemical analysis
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