Assessment of MiR-191-5p as a Predictive Marker of Early Diabetic Sensorimotor Polyneuropathy in Patients with Newly Diagnosed Type 2 Diabetes Mellitus | ||||
| Zagazig University Medical Journal | ||||
| Article 9, Volume 30, Issue 1.4, May and June 2024, Page 100-110 PDF (1.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zumj.2023.253034.3030 | ||||
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| Authors | ||||
Nearmeen Rashad   1; Hoda Afifi2; Mohammed Hanafy Aly Ghonemy3; ahmad sallam4; Radwa Mahmoud Al-Sayed5; Nancy Abdelhamid Mohammad6; Dina Issa7
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| 1ZAGAZIG UNIVERSITY | ||||
| 2Ain Shams University | ||||
| 3Neurology department ,faculty of medicine ,Zagazig university,Zagazig ,egypt | ||||
| 4clinicalpathology department | ||||
| 5physiology department, Faculty of medicine, Zagazig University, Egypt. | ||||
| 6Faculty of medicine, neurology department | ||||
| 7helwan university | ||||
| Abstract | ||||
| Abstract Background: Diabetic sensorimotor polyneuropathy (DSPN) is the most common complication of type 2 diabetes mellitus (T2DM) and the major cause of nontraumatic lower limb amputation. We aimed in the current research to investigate miR-191-5p as a noninvasive predictive biomarker of DSPN among patients recently diagnosed with T2DM in associations with clinical and electrophysiological tests. Methods: We conducted fifty cases recently diagnosed with T2DM and 50 healthy control subjects. contributors were evaluated by clinical and laboratory investigations in addition to nerve conduction studies. circulatory miR-191-5p assessed using the Real-Time PCR method. Results: miR-191-5p levels were lower in patients with DSPN compared to patients without DSPN and controls. Remarkably, miR-191-5p levels were significantly negatively correlated with cardiometabolic risk factors and neuropathy scores; Neuropathy Disability Score (NDS), Neuropathy Symptom Score (NSS), and Toronto Clinical Scoring System (TCSS), p ˂0.001*. The linear regression test revealed that TCSS, HbA1c, and LDL were the main independent variables against Mir-191-5p levels in patients with DSPN, p ˂0.001*. To assess the predictive values of miR-191-5p we applied the ROC curve the cutoff values of miR-191-5p as a predictive marker for DSPN was (0.559), with a sensitivity of (95%) and a specificity of (98.7%), with the AUC was 0.958 (0.922-0.993), p ˂0.001*). Conclusion: circulating miR-191-5p was significantly downregulated in patients recently diagnosed with T2DM, more specifically in patients with DSPN, and it could be used as a biomarker for predicting diabetes and DSPN. | ||||
| Keywords | ||||
| Diabetic sensorimotor polyneuropathy; microRNA; Neuropathy Disability Score; Neuropathy Symptom Score; Toronto Clinical Scoring System | ||||
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