BLASTOCYSTIS SUBTYPE 3 AMONG ADOLESCENTS WITH GASTROINTESTINAL SYMPTOMS IN FAYOUM GOVERNORATE, EGYPT | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 8, Volume 53, Issue 3, December 2023, Page 467-474 PDF (1.04 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2023.331732 | ||||
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| Authors | ||||
| Faten Ahmed Mahmoud Mohamed1; KHOLOUD ADEL KHALIL2 | ||||
| 1Department of Medical Parasitology, Faculty of Medicine, Fayoum University | ||||
| 2Department of Public Health, Damanhour National Medical Institute, Damanhour, Egypt | ||||
| Abstract | ||||
| Blastocystis is a common polymorphic protozoan infecting human intestine with a zoonotic potential. Despite the unclear pathogenicity, its existence may be linked to a wide range of gastrointestinal disorders. The present cross sectional study was conducted to analyze Blastocystis subtypes among 110 adolescents suffering from disturbing GIT symptoms in stool samples. Positive samples (21 (19.1%)) by microscopy were subjected to DNA isolation and subtyped by Sequence Tagged Site (STS)-PCR analysis, which successfully subtyped 10 (62.5%) of 16 isolates. The dominant Blastocystis subtype was ST3 in 6 (40%) samples followed by 3 (30%) for ST2, but one sample (10%) was amplified for ST1. The results revealed significant association between nausea and vomiting and Blastocystis infection. However, there was no difference between the Blastocystis subtypes and demo-clinical characteristics. | ||||
| Keywords | ||||
| Blastocystis; Sequence-Tagged Sites; PCR; Subtyping; Microscopy | ||||
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