EFFECTS OF INTRAMUSCULAR INJECTION OF PLATELET-RICH PLASMA ON LARVAL BURDEN OF TRICHINELLA SPIRALIS IN EXPERIMENTALLY INFECTED MICE | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 17, Volume 53, Issue 3, December 2023, Page 559-568 PDF (1.41 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jesp.2023.331741 | ||||
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Authors | ||||
FATMA H. SHALAAN1; TAHANY A. A. AL-ATTAR1; SHEREEN F. MAHMOUD2; HUDA IBRAHIM ABD-ELHAFIZ3; YOUSRA A. AL-GHALBAN4; MONA F. FAHEEM5 | ||||
1Department of Medical Parasitology | ||||
2Department of Pathology | ||||
3Department of Clinical Pharmacology | ||||
4Department of Public Health and Community Medicine | ||||
5Department of Basic Medical Sciences, Faculty of Medicine, King Salman International University, South Sinai Governorate, Egypt | ||||
Abstract | ||||
Trichinellosis (= trichinosis) is widespread food-borne zoonotic parasitosis caused by the nematode Trichinella spiralis triggering skeletal muscle injury. This study identified the therapeutic effects of platelet-rich plasma (PRP) as a safe choice in muscular phase of mice experimental infected with T. spiralis. Ninety male mice were divided into three main groups, GI: Negative control, GII: Positive control and GIII: Trichinella infected PRP treated. All mice were sacrificed on 14th day post infection (initiation of muscle cell invasion), on 21st day (early stage of nurse cell formation), on 28th day (formation of most nurse cells was completed), and on 35th day PI (convalescent period). The parasitological examination showed a significant reduction in the number of larvae detected in the infected PRP-treated ones 35th PI. Histopathological and immunohistochemical of vascular endothelial growth factor, caspase 3 analyses coincided with parasitological findings, on 35th day PI as well. There was a significant improvement in the in-flammatory cells with fewer muscle fibers degradation, indicating that PRP injection in trichinellosis muscular phase improved inflammatory reaction and reduce T. spiralis larval burden. | ||||
Keywords | ||||
Trichinellosis; Platelet-rich plasma; Vascular endothelial growth factor; Caspase 3 | ||||
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