Potential protective role of spirulina algae and/or aminoguanidine on carbon tetrachloride induced liver fibrosis in rats. | ||||
| Journal of Scientific Research in Science | ||||
| Volume 40, Issue 2, 2023, Page 153-170 PDF (1.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jsrs.2023.331820 | ||||
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| Authors | ||||
mona fathy shawky   1; M. I. Heibashy2; S. R. Wahba1
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| 1Zoology Department, Faculty of Women for Arts, Science and Education , Ain Shams University, Cairo, Egypt. | ||||
| 2Biological Applications Department, Radioisotopes Applications Division, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt. | ||||
| Abstract | ||||
| The present study focuses on fibrotic effects on rat liver tissue induced by carbon tetrachloride (CCl4) injection and the possible protective effects of spirulina algae (SP) and/or aminoguanidine (AG) on induced histological and biochemical changes. To achieve this goal, a comparison was conducted between control and fibrotic groups (60 rats each). Fibrotic rats model was induced by the injection with an intraperitoneal dose of 2.0 ml/kg body weight CCl4 twice/ week for four weeks to induce fibrosis. Control group was divided into four subgroups (n=15) as follows: control given saline through orogastric tube; SP administered with SP at a dose of 1g/kg/b.wt; AG administered with AG at a dose of 100 mg AG/kg/b.wt; double treatment group with SP+AG at the aforementioned doses (1g/kg/b.wt of SP+ 100mg/kg/b.wt of AG). Fibrotic groups were similarly subdivided into 4 subgroups (n=15): fibrotic non-treated; SP; AG and SP + AG as a mixture treated subgroups. The study extended for 3 time intervals, 2, 4 and 6 weeks (n=5/ intervals). Histological alterations in liver tissue of fibrotic rats included hepatocytes degeneration with pyknotic nuclei and connective tissue fibers proliferation using haemtoxylin & eosin and silver impregnation technique for investigations. Biochemical results showed significant (P˂0.05) increase in serum levels of α-fetoprotein (AFP), carcino embryonic antigen (CEA), tumor necrosis factor-alpha (TNF-α). On the other hand, significant (P˂0.05) decrease in activity of cytochrome P450 content (CYP-450) was recorded in fibrotic rats compared with the normal controls. When fibrotic subgroups were treated with SP and/or AG, considerable protective effects in previous biochemical and histological parameters were recorded especially in mixture treated group. | ||||
| Keywords | ||||
| Aminoguanidine; Carbon tetrachloride; Fibrosis; Spirulina; Rats | ||||
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