Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)
El Wakad, A., Moukarab, D., Abdelhalim, E. (2023). Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study). EKB Journal Management System, (), 44-54. doi: 10.21608/ueccj.2023.256886.1009
Ahmed Amr El Wakad; Dalia Ali Ahmed Moukarab; Enas Fathelbab Abdelhalim. "Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)". EKB Journal Management System, , , 2023, 44-54. doi: 10.21608/ueccj.2023.256886.1009
El Wakad, A., Moukarab, D., Abdelhalim, E. (2023). 'Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)', EKB Journal Management System, (), pp. 44-54. doi: 10.21608/ueccj.2023.256886.1009
El Wakad, A., Moukarab, D., Abdelhalim, E. Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study). EKB Journal Management System, 2023; (): 44-54. doi: 10.21608/ueccj.2023.256886.1009

Evaluation of Antimicrobial Efficacy of Silver Nanoparticles and Chitosan Nanoparticles against Enterococcus Faecalis and Candida Albicans Biofilm (An in vitro Study)

Upper Egypt Critical Care Journal
Articles in Press, Accepted Manuscript, Available Online from 27 December 2023  XML PDF (574.98 K)
Document Type: Original Article
DOI: 10.21608/ueccj.2023.256886.1009
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Authors
Ahmed Amr El Wakad email orcid 1; Dalia Ali Ahmed Moukarab2; Enas Fathelbab Abdelhalim3
1Doctorate Candidate of Endodontics, Faculty of Dentistry, Minia University. Assistant Lecturer of Endodontics, Faculty of Oral and Dental Medicine, Future University in Egypt
2Associate Professor of Endodontics, Head of Endodontic Department Faculty of Dentistry, Minia University
3Professor of Fixed Prosthodontics Faculty of Dentistry, Minia University
Abstract
Aim: antibacterial activities of silver nanoparticles and chitosan nanoparticles against Enterococcus Faecalis and Candida Albicans biofilm in an in- vitro model
Material and methods:
Forty extracted human single-rooted permanent teeth were included in the present study. All samples were prepared up to size Revo S (AS 40/0.06), and autoclaved for 20 minutes at 121°C then divided into 5 equal groups (n=8); negative control (no further treatment was preformed) and positive control ( only microbial inoculation was preformed) , all tested groups were inoculated then further treated with irrigation solutions group I (treated with nanosilver particles) , group II ( treated with chitosan nanoparticles ) and group III (treated NaOCl/ EDTA). For all specimens microbial analysis was performed.
Results: The higher median value of bacterial count in Log CFU ml -1 in Positive control was 6.20 (5.99-6.55), followed by Group I (Nano- silver) was 1.38 (0.51-1.88), then Group II (Nano-chitosan) was 1.38 (1.04-1.55), followed by Group III (Sodium hypochlorite) was 0.73 (0.34-1.37), then Negative control was 0 (0-0). Kruskal Wallis test revealed a statistically significant difference between groups (P=0.00). Mann-Whitney test revealed no significant difference between groups I and II, with p-value (p>0.05).
Conclusion: Nano silver and nano chitosan particles are effective microbial agents against E. faecalis and Candida albicans however; sodium hypochlorite has a significantly superior antimicrobial efficacy.
Keywords
Silver Nanoparticles; Chitosan Nanoparticles; Enterococcus Faecalis; Candida Albicans; Biofilm
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