MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats
Ahmed Mohamed, M., Abdel Zaher, A., Ahmed, H., Abdeen Radwan, H., Hishmat, A. (2023). MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats. EKB Journal Management System, 34(4), 54-64. doi: 10.21608/mjmr.2023.255155.1564
Mennatallah Mahmoud Ahmed Mohamed; Azza Mohamed Abdel Zaher; Hala Mohamed Ahmed; Hanaa Yhia Abdeen Radwan; Asmaa Mohammed Hishmat. "MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats". EKB Journal Management System, 34, 4, 2023, 54-64. doi: 10.21608/mjmr.2023.255155.1564
Ahmed Mohamed, M., Abdel Zaher, A., Ahmed, H., Abdeen Radwan, H., Hishmat, A. (2023). 'MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats', EKB Journal Management System, 34(4), pp. 54-64. doi: 10.21608/mjmr.2023.255155.1564
Ahmed Mohamed, M., Abdel Zaher, A., Ahmed, H., Abdeen Radwan, H., Hishmat, A. MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats. EKB Journal Management System, 2023; 34(4): 54-64. doi: 10.21608/mjmr.2023.255155.1564

MicroRNAs molecular biomarkers for determination of drowning death in relation to postmortem interval in different organs of albino rats

Minia Journal of Medical Research
Article 7, Volume 34, Issue 4, October 2023, Page 54-64  XML PDF (1.44 MB)
Document Type: Original Article
DOI: 10.21608/mjmr.2023.255155.1564
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Authors
Mennatallah Mahmoud Ahmed Mohamed email 1; Azza Mohamed Abdel Zaher2; Hala Mohamed Ahmed1; Hanaa Yhia Abdeen Radwan1; Asmaa Mohammed Hishmat1
1Forensic Medicine and Clinical Toxicology Depatment, Faculty of Medicine, Minia University, Minia, Egypt
2Pathology Department, Faculty of Medicine, Minia University, Minia, Egypt
Abstract
Background: Forensic professionals have a crucial responsibility when estimating the postmortem interval (PMI), particularly in criminal situations. Drowning is a deadly asphyxiation incident brought by extended submersion in a liquid. Diagnosing death toll from drown remains the most complex challenge for forensic pathologists. This work aimed to determine drowning deaths in relation to PMI using histopathological changes and the expression of microRNA-23b-3p and microRNA-381-3p in brain and lung tissues. Methods: In this study, thirty adult albino rat models were used. Three groups were created out of them (0, 12, 24 hrs. after death), each of which contains 10 rats. Brain and lung tissues from each rat were taken out and divided into two pieces. The first was prepared for histopathological analysis, and the second portion was prepared for the analysis of microRNA expression by RT-PCR. Results: The brain tissues showed pyknotic nuclei of the neurons with mild vacuolation at the time of death, which progressed with an increased postmortem interval to marked vacuolation. While lung tissues showed overdistention of the alveoli, which became marked with an increased postmortem interval with marked dissolution of interstitial cells at 24 hrs  postmortem. MicroRNA-23b-3p and microRNA-381-3p levels showed a significant difference in brain and lung tissues at different postmortem intervals. Conclusion: MicroRNA-23b-3p and MicroRNA-381-3p expression could be helpful as new biomarkers for the determination of drowning death in relation to time since death.
Keywords
Drowning; MicroRNA-23b-3p; MicroRNA-381-3p
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