Spiramycin-chitosan Nanoparticles Decline Parasite Burden and Renovate Patent Histopathological Changes in Liver and Lung in Mice Experimentally Infected with Acute Toxoplasmosis | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Volume 55, Issue 4, July and August 2024, Page 1151-1164 PDF (1.4 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.253903.1708 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Dalia Osama1; Nadia El-Dib2; Hala khattab1; Hassan Ibrahim3; mona khater2; Ehab Elmahallawy4; Ashraf Mohamed Abdel Khalek Barakat   5
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| 1Department of Medical Parasitology, Research Institute of Ophthalmology, Cairo, Egypt | ||||
| 2Department of Medical Parasitology, Faculty of Medicine - Cairo University, Cairo, Egypt. | ||||
| 3Textile Research and Technology Institute, National Research Centre, 33 El Bohouth st., Dokki, P.O.12622. Cairo, Egypt | ||||
| 4Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University | ||||
| 5Prof. Dr. Zoonotic Diseases Department | ||||
| Abstract | ||||
| The present study was designed to evaluate the use of nanoparticles in improving the antiparasitic effect of Spiramycin, Spiramycin loaded on Carboxymethyl chitosan nanoparticles (CMC Np), ) on experimental toxoplasmosis. Different doses of the drugs under study were assessed using parasitological and histopathological investigations. For this purpose, a total of 38 female Swiss albino mice were divided as follows; Group 1 (5 mice) as a negative control group (non-infected, non-treated group). Group 2 (5 mice) as a positive control group (infected, non-treated group); Group 3: In this group, 28 mice were infected, and treatment started after 10-15 days post-infection for 1 week on a daily basis. The third group was subdivided into four subgroups (7 mice for each), which were treated as follows; subgroup (3a); the infected mice treated with Spiramycin alone in a dose of 100 mg/ kg /day orally, sub group (3b); the infected mice treated with CMC Np orally. Sub group (3c); the infected mice treated with Spiramycin loaded on CMC Np of concentration of 0.35 gm/100 ml H2O (low dose-LD) orally. Subgroup (3d); the infected mice treated with Spiramycin loaded on CMC Np of concentration of 0.70 g/ 100 ml H2O (High dose-HD) orally. At the end of the experiment, liver and lung were dissected for detection of the parasite burden and the histopathological examination of the tissues was carried out to allocate the histopathological findings in these organs and detection of tissue cysts. Remarkably, a noticeable decrease in parasitic load was stated pooled with renovation of histopathological alterations were prominent with treated groups in association with infected non-treated group. | ||||
| Keywords | ||||
| Acute toxoplasmosis; Spiramycin; Carboxymethyl chitosan nanoparticles | ||||
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