Assessment of Commercial Bivalent Inactivated Newcastle Disease Virus and Infectious Bronchitis Virus Vaccines against Prevalent Isolates in Egypt | ||||
Journal of Veterinary Medical Research | ||||
Article 12, Volume 30, Issue 2, December 2023, Page 130-136 PDF (1.2 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jvmr.2024.241881.1093 | ||||
View on SCiNiTO | ||||
Authors | ||||
Wahid Hussein El-Dabae 1; Hala Mahmoud Attya 2; Marwa Fathy Elsayed 3; Reem Ahmed Soliman 3; Moustafa Ahmed Zaghlool 4; Mounir Mohamed El-Safty 5 | ||||
1department of microbiology and immunology, veterinary research institute, national research center | ||||
2SPF egg department Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Cairo 11381, Egypt | ||||
3SPF department Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Cairo 11381, Egypt | ||||
4SPF departmentCentral Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Cairo 11381, Egypt | ||||
5SPF department Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Cairo 11381, Egypt | ||||
Abstract | ||||
Newcastle disease (NDV) and infectious bronchitis (IB) viruses are the most infectious and serious diseases on poultry flocks Egypt. Several types of bivalent inactivated NDV and IB vaccines are used to prevent and decrease incidence of both viruses but efficacy of these vaccines should be monitored periodically to understand the main causes of those breaks. In the current study nine bivalent inactivated NDV and IB vaccines were randomly selected from different batches during routine work at central laboratory for evaluation of veterinary biologics in 2023. Quality control of nine vaccines was applied according to Egyptian standard regulations for evaluation of veterinary Biologics. Completion of inactivation test of nine vaccines for both NDV and IB viruses revealed negative haemaagglutination test for NDV and normal appearance of embryo of IB virus when inoculation in SPF eggs. Immunization of one week old SPF chickens of nine selected vaccines showed neither clinical signs nor mortalities. Collection of serum samples from all tested vaccines on 3rd and 4th weeks post vaccination showed variable immune response against NDV using Haemagglutination inhibition test and ELISA test against IB virus. Challenge trial of tested vaccines showed variable percent of protection (50% to 100%). Virus shedding in SPF eggs on 3rd, 5th, 7th and 10th days post challenge revealed significant amount of virus in oropharengeal swabs. This study showed validation of some bivalent inactivated NDV and IB vaccines batches in vaccination programs and invalidation of other batches for use. | ||||
Keywords | ||||
Bivalent NDV and IBV Inactivated Vaccines; Genotype; Infectious Bronchitis Virus; Newcastle Disease Virus; Quality Control | ||||
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