Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn
Harb, I., Osman, A., Mostafa, A., Hegab, Z., Sabry, M., Kalifa, S., Maher, A. (2024). Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn. EKB Journal Management System, 67(7), 421-434. doi: 10.21608/ejchem.2024.234184.8558
Inas Harb; Afaf Osman; Abeer Mostafa; Zeinab Hegab; Marwa Mohamed Sabry; Sara Kalifa; Amr Maher. "Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn". EKB Journal Management System, 67, 7, 2024, 421-434. doi: 10.21608/ejchem.2024.234184.8558
Harb, I., Osman, A., Mostafa, A., Hegab, Z., Sabry, M., Kalifa, S., Maher, A. (2024). 'Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn', EKB Journal Management System, 67(7), pp. 421-434. doi: 10.21608/ejchem.2024.234184.8558
Harb, I., Osman, A., Mostafa, A., Hegab, Z., Sabry, M., Kalifa, S., Maher, A. Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn. EKB Journal Management System, 2024; 67(7): 421-434. doi: 10.21608/ejchem.2024.234184.8558

Resuscitation Using Hypertonic Saline, Effect on Muscle Performance and Wound Healing in A Rat Model of Burn

Egyptian Journal of Chemistry
Volume 67, Issue 7, July 2024, Page 421-434  XML PDF (3.25 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2024.234184.8558
View on SCiNiTO View on SCiNiTO
Authors
Inas Harb1; Afaf Osman email 2; Abeer Mostafa3; Zeinab Hegab4; Marwa Mohamed Sabry4; Sara Kalifa5; Amr Maher2
1Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Egypt
2Department of Medical Pharmacology, Faculty of Medicine, Cairo University
3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
4Department of Histology, Faculty of Medicine, Cairo University
5Department of Pathology, Faculty of Medicine, Cairo University
Abstract
Muscle wasting is a serious burn complication. The possible protective impact of hypertonic saline vs. ringer lactate on muscle wasting and wound healing in a rat burn model was investigated.
Rats were divided into; control rats received normal saline, untreated burn rats, ringer lactate treated burn rats & hypertonic saline treated burn rats received normal saline, ringer lactate or hypertonic saline, respectively after induction of burn. After 18 hours, serum levels of substance P, tumor necrosis factor- alpha (TNF-α), interleukin-1beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), and interleukin-10 (IL-10) were measured. Wound healing was assessed after 2, 4, and 6 weeks. At the end of the 6th week, gastrocnemius muscle weight and performance, transforming growth factor (TGF-β), collagen type I, forkhead box transcription factors O (FOXO) gene expression and histopathological examination were done. Hypertonic saline treatment significantly lowered substance P, TNF-α, IL-1β, IL-6, IL-2 with elevated IL 10, improved muscle weight and performance, improved wound healing, reduced TGF ß, collagen I and FOXO genes compared with ringer lactate treatment. Histopathological findings supported these results.
Early use of hypertonic saline in burn showed a more promising effect on the improvement of muscle performance and skin regeneration than ringer lactate solution does.
Keywords
Hypertonic saline; ringer lactate; muscle performance; wound healing; burn
Statistics
Article View: 224
PDF Download: 20