Physiological Responses and Histopathological Indices against Acrylamide Toxicity Treated with Metformin and Propolis in Clarias gariepinus | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 5, Volume 28, Issue 1, January and February 2024, Page 105-130 PDF (1.19 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2024.336220 | ||||
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| Author | ||||
| Bassem E. Elaraby et al. | ||||
| Abstract | ||||
| Acrylamide (AA), a substance extensively used in business, medical research, and waste management facilities, is thought to cause cancer in people. The Clarias gariepinus vertebrate fish model was used in the current investigation to assess the stress biomarkers, hematologic, and histopathological indices following exposure to (AA). Two hundred healthy fish of C. gariepinus of both sexes wereclassified into four groups: the first is the negative control (CO) (without acrylamide); the second is the positive control (CA) (treated with acrylamide only) using 15mg/ l concentrations of (AA); the third is (AM) which contains (AA) plus metformin (MeFo) applied to treatment with (AA) at (15mg/ l) and (MeFo) at 3g/ l, and the fourth is (AP) which contains (AA) plus propolis (PPo) applied to the treatment with (AA) at (15mg/ l) and (PPo) at 3g/ l. The hematological parameters, cortisol, glucose, and lactate dehydrogenase (LDH) were analyzed. The current data showed a significant increase in white blood cells, hemoglobin, hematocrit, cortisol, glucose, and LDH in (CA) groups when compared with (CO) group. A histopathological analysis of the kidney, spleen, and liver was performed. The groups (CA), (AM), and (AP) had noticeable structural changes in the kidney, spleen, and liver in the form of congestion, apoptosis, and vacuolations compared to the (CO) group. Administration of (AA) resulted in serious liver, spleen, and kidney damage for C. gariepinus catfish. However, (PPo) and (MeFo) exhibited anti-toxicity and improved kidney, spleen, and liver functions. The findings of the current study demonstrated that extensive and regular usage of (AA) could endanger the existence and welfare of organisms, particularly fish, in aquatic environments. | ||||
| Keywords | ||||
| Acrylamide; Histopathological; Metformin; Physiological; Propolis | ||||
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