Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt
Elbestawy, A., Elnagar, A., Saad, A., Khalifa, E. (2024). Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt. EKB Journal Management System, (), -. doi: 10.21608/mjvm.2023.248574.1021
Ahmed Ragab Elbestawy; Ahmed Ragab Elnagar; Adel Hassan Saad; Eman Khalifa. "Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt". EKB Journal Management System, , , 2024, -. doi: 10.21608/mjvm.2023.248574.1021
Elbestawy, A., Elnagar, A., Saad, A., Khalifa, E. (2024). 'Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt', EKB Journal Management System, (), pp. -. doi: 10.21608/mjvm.2023.248574.1021
Elbestawy, A., Elnagar, A., Saad, A., Khalifa, E. Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt. EKB Journal Management System, 2024; (): -. doi: 10.21608/mjvm.2023.248574.1021

Isolation and molecular characterization of velogenic Newcastle disease virus genotype VII.1.1 from commercial broilers and wild birds in Matrouh governorate, Egypt

Matrouh Journal of Veterinary Medicine
Articles in Press, Accepted Manuscript, Available Online from 13 January 2024  XML
Document Type: Research articles
DOI: 10.21608/mjvm.2023.248574.1021
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Authors
Ahmed Ragab Elbestawyorcid 1; Ahmed Ragab Elnagar email 2; Adel Hassan Saad3; Eman Khalifaorcid 4
1Faculty of veterinary medicine Damanhour university
2Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Matrouh University, Marsa Matrouh, Egypt
3Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Matrouh University, Marsa Matrouh, Egypt
4Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Fuka 51744, Matrouh, Egypt
Abstract
Velogenic Newcastle disease virus (vNDV) genotype VII.1.1 continues to cause severe economic losses in chicken flocks since its first detection in 2011. In this study, pooled samples from 36 diseased commercial broiler chicken flocks (vaccinated against ND) which were suspected to be infected with vNDV and 15 pooled cloacal swabs from 6 wild bird species in Matrouh governorate were tested for vNDV genotype VII.1.1. The results showed that 5 broiler samples (5/36 = 13.8%) and 2 cloacal swabs from wild marbled ducks (2/15 = 13.3%) were positive by real-time RT-PCR and 6/7 isolates had mixed viral infections. F gene partial sequence analysis of 2 vNDV isolates (broiler origin, with a GenBank accession No. OR778089) and (wild marbled duck origin, with a GenBank accession No. OR778090), revealed 97.9-100% similarity with previous Egyptian isolates of vNDV-VII.1.1. Furthermore, the intracerebral pathogenicity index score of an isolate of duck origin (no. 44) was 1.82. In conclusion, wild marbled ducks could be an important source of transmission of vNDV-VII.1.1 to commercial broiler flocks. Furthermore, the isolation of vNDV-VII.1.1 from vaccinated commercial broiler flocks necessitates the use of more genetically and antigenically matched vaccines for effective control of this devastating viral infection.
Keywords
vNDV-VII.1.1; Fusion Protein; Matrouh
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