Serum activin-A as a potential biomarker for early stage ulcerative colitis (UC): An immunological and molecular evaluation study using murine animal model | ||||
| Journal of Applied Molecular Biology | ||||
| Volume 2, Issue 1 - Serial Number 2974, January 2024, Page 52-70 PDF (1.42 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jamb.2023.219216.1009 | ||||
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| Authors | ||||
khadiga abdelrhman aboelail   1; Amr M. M Abdelfattah2; Mahmoud Rushdi Abd Ellah3; Nashwa Esmat Waly4; Khaled M. A Hassanein5
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| 1Animal medicine, Veterinary medicine, Assiut university | ||||
| 2Dean for graduate studies and research Molecular biology institute. animal medicine, veterinary medicine, assiut university | ||||
| 3head of animal medicine department, vererinary medicine, assiut university | ||||
| 4Animal medicine department,veterinary medicine, assiut university | ||||
| 5pathology department, veterinary medicine, assiut university | ||||
| Abstract | ||||
| Ulcerative colitis (UC), a form of inflammatory bowel disease (IBD), is a chronic tissue-destructive disorder of the colon. Current laboratory diagnostic assays depend on evaluation of calprotectin that boost with the advancement of the diseases. The present study aimed to evaluate activin-A as a potential alternative marker for early detection of ulcerative colitis (UC). Experimental murine models of short-term versus long-term dextran sodium sulphate (DSS)-induced UC were developed and assessed histopathologically for evaluation purposes. Immunoassays were adopted for evaluation of both activin-A and calprotectin levels in collected serum and fecal specimens using commercial ELISA kit. Colon tissue specimens were homogenized and used for evaluation of activin-A and calprotectin gene expression using quantitative real-time PCR. The results revealed significant increases in serum and fecal activin-A in both short-term and long-term colitis models while there was a significant increase of fecal calprotectin only in long-term UC. Evaluation of gene expression revealed significant upregulation of activin-A in short-term but not in long-term UC model, whereas calprotectin expression showed significant upregulation in long-term but not in short-term of UC model. Interestingly, strong positive correlation was evident between serum, fecal and gene expression of activin-A in both short-term and long-term UC models. On the other hand, calprotectin showed negative correlation between serum and fecal levels in long-term model and positive correlation between serum and gene expression levels in short-term UC. In conclusion, while fecal calprotectin is a good indicator for late stage UC, serum activin-A is a potential biomarker that promote early prognosis of UC. | ||||
| Keywords | ||||
| Activin-A; DSS; Ulcerative colitis; Immunoassay; Gene expression | ||||
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