Effect of Vitamin D overdose on VDR, TRPV6 and CYP3A11 genes expression, biochemical tests and histopathological legions in Albino mice | ||||
| Journal of Applied Molecular Biology | ||||
| Volume 2, Issue 1 - Serial Number 2974, January 2024, Page 71-92 PDF (2.42 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jamb.2023.230157.1013 | ||||
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| Authors | ||||
Amr Abd el nasser Abd el fatah  1; Asmaa Abdo Abdelrahman2; Bahaa El-Din El-Sayed Abdel-Fattah3; Rania Mohammed Ewida4; Ameer Effat M. Elfarash5
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| 1Applied Biotechnology Dept., Molecular Biology Research & Studies Institute. Assiut University, Assiut, Egypt | ||||
| 2Histology Dept., Faculty of Veterinary. Assiut University Assiut, Egypt. | ||||
| 3Department of Genetics- Facully of Agriculture - Assiut University- Assuit Egypt | ||||
| 4Faculty of Veterinary Medicine, New VallDepartment of Food Hygiene,Safety and Technology Faculty of Veterinary Medicine,New Valley Universityey University, New Valley, Egypt | ||||
| 5Department of Applied Biotechnology, Molecular Biology Researches & Studies Institute, Assiut. Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt. School of Veterinary Medicine, Badr University in Assiut, Egypt. | ||||
| Abstract | ||||
| Due to the spread of corona virus, many doctors’ advice people to take vitamin D to prevent catching corona virus. But some people used it carelessly without keeping out the consequences of toxicity. For this reason, this study concerns on the impact of vitamin D overdose in mice on the expression of different important genes (VDR, TRPV6 and CYP3A11 genes), histopathological legions, and biochemical properties. Forty male Albino mice, each male weighs 35-40 mg, were divided randomly to four group each group contain 10 mice. Each group were treated daily with a different concentration of Vitamin D for 8 weeks by a stomach tube. The Groups were control (without Vitamin D), low dose (1um of Vitamin D /ml), intermediate dose (2 um of Vitamin D /ml), high dose (3 um of Vitamin D /ml). After 8 weeks, the mice were slaughter and different tissues were examined. Results showed that vitamin D overdose caused up regulation in the VDR gene expression, and down regulation in both TRPV6 and CYP3A11 genes. Moreover, vitamin D overdose caused significant histopathological changes that were observed in different tissues as stomach, heart, lung and kidney. Biochemical properties were investigated to test the liver function by measuring the ALT, and AST enzymes. While the Kidney function were examined by measuring BUN, vitamin D and calcium levels. The biochemical tests illustrated that vitamin D overdose caused raised level of total calcium, BUN, ALT and AST enzyme in blood. These signs referred to damage of kidney and liver relatively. | ||||
| Keywords | ||||
| vitamin D; VDR; TRPV6 and CYP3A11 genes; histopathological lesions; biochemical effects | ||||
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