Comparative Clinicopathological studies on the anti-inflammatory effect of Ashwagandha and Diclofenac in rats | ||||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||||
| Articles in Press, Accepted Manuscript, Available Online from 17 January 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scvmj.2024.261413.1158 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Haidy Galal Abdel-Rahman   1; omnia G Badry2; Osama Abdalla3; Amina Ali Dessouki 4
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| 1Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt | ||||
| 2Free Veterinarian, Ismailia, Egypt | ||||
| 3Professor of Clinica Pathology Faculty of Veterinary Medicine, Suez Canal University | ||||
| 4Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt | ||||
| Abstract | ||||
| Arthritis is a painful inflammatory illness, characterized by joint destruction and disability. Non-steroidal anti-inflammatory drugs (NSAIDs) are applied extinsively as pain relievers, however, their prolonged administration can result in undesirable side impacts on various body organs. Ashwagandha (Withania somnifera) has drew researchers’ consideration by virtue of its several medical uses. The current study investigated the anti-inflammatory action of ashwagandha (Ash) versus that of diclofenac sodium (Dic) via the evaluation of paw thickness, hematological, biochemical and inflammatory parameters and histopathological alteration in rats. Rats were distributed into 6 groups, kept for 21 days: control; received normal saline orally, Ash group; orally treated with Ash (200 mg/kg body weight), Dic group; orally treated with Diclofenac sodium (2 mg/kg b.wt), Arthritic (Arth); rats were subjected to sub-plantar injection of 0.1 ml formalin (2.5%) at right hind paw on day 1 and received normal saline orally, Arth+Ash; arthritis was induced as explained in Arth group then treated with Ash (at the same dose as Ash group) orally, Arth+Dic; arthritis was induced as explained in Arth group then treated with Dic (at the same dose as Dic group) orally. Paw thickness was significantly increased in Arth rats than the control ones at various estimated times along the experiment, however, significant decline was recorded in Arth+Ash and Arth+Dic groups. Upon completion of the research (21 days), blood and tissue specimens were harvested. Hematological outcomes announced normocytic normochromic anemia in Arth rats, and this effect was alleviated upon administration of Ash to Arth rats. While, Dic had negative drawbacks on erythrogram. Serum ALT, urea, creatinine, IL-6, CRP, COX2 and hepatic TOC exhibited substantial increment along with substantial decrement in serum IL-10 and hepatic TAC in Arth group. Meanwhile, Arth+Ash and Arth+Dic groups showed significant improvement in these parameters in comparison to Arth group. In addition, our histopathological assessment illustrated the adverse sequel of Dic on miscellaneous rat’s body tissues and that Ash displayed invulnerable action. Lastly, oral administration of W. sominefra extract to rats with arthritis induced by formalin, remarkably decreased paw thickness and alleviated unfavorable modifications in all measured variables in a way better than diclofenac. | ||||
| Keywords | ||||
| Ashwagandha; arthritis; diclofenac; inflammation; rats | ||||
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