Linking 5’-promoter region of Cdx2 VDR polymorphism and serum vitamin D association in female genital tuberculosis. | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 17 January 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.250347.1672 | ||||
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| Authors | ||||
Shyam Pyari Jaiswar  1; Ummehani Siddiqui2; Dr. Apala Priyadarshini3; Amita Jain4
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| 1Department of Obstetrics & Gynecology, King George's Medical University, Lucknow,U.P | ||||
| 2Department of Obstetrics & Gynecology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India | ||||
| 3Department of Obstetrics & Gynecology, All India Institute of Medical Sciences, Raebareli (229405), Uttar Pradesh, India | ||||
| 4Dept. of Microbiology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India | ||||
| Abstract | ||||
| Background: Globally, genital tuberculosis (GTB) is a substantial cause of female infertility. The vitamin D receptor (VDR), a member of the nuclear receptor superfamily, mediates the immunological function of vitamin D3, activates macrophages, and vitamin D deficiency has been linked to FGTB risk. Objective: The aim of this study is to assess the relationship between risk of FGTB and the vitamin D receptor gene polymorphism (Cdx2) and serum vitamin D level. Methods: 150 confirmed FGTB cases and 150 healthy controls were recruited. Serum vitamin D level was measured by ELISA. Genomic DNA was extracted and the genotyping of VDR-Cdx2 polymorphism was performed by T-ARMS-PCR. Results: Serum vitamin D levels were significantly lower among FGTB patients. The frequency of A allele was 68% in FGTB and 48.6% in control; A allele was significantly associated with increased risk of FGTB [OR = 2.24; 95 % confidence interval [CI]; p< 0.0001] however, the frequency of G allele (32% in FGTB and 51.3% in control; G allele did not show significant risk of FGTB [OR = 1.10; 95 % confidence interval [CI]; p = 0.67]. A significant association was found between VDR Cdx-2 AA (p <0.001) genotype and vitamin D level. Conclusion: Genotype frequencies of VDR gene polymorphism and Vit D were found significantly association leads, VDR dysfunction, could increase FGTB risk. Understanding the synergism between VDR polymorphism and vit D in FGTB will be important to identifying the new prognostic tool and target for therapy in vit D deficient individuals. | ||||
| Keywords | ||||
| Genital Tuberculosis; Caudal-type homeobox2; Single-nucleotide polymorphism; VDR genotyping | ||||
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