Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates
Mohammed, A., Hassan, E., Hassan, M., Reda, A., Elghazaly, S., Mohammed, S. (2024). Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates. EKB Journal Management System, 5(1), 260-269. doi: 10.21608/mid.2024.255029.1711
Ayat H. Mohammed; Ehsan A Hassan; Mona A. Hassan; Ahmed Reda; Shrouk M. Elghazaly; Shereen M. Mohammed. "Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates". EKB Journal Management System, 5, 1, 2024, 260-269. doi: 10.21608/mid.2024.255029.1711
Mohammed, A., Hassan, E., Hassan, M., Reda, A., Elghazaly, S., Mohammed, S. (2024). 'Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates', EKB Journal Management System, 5(1), pp. 260-269. doi: 10.21608/mid.2024.255029.1711
Mohammed, A., Hassan, E., Hassan, M., Reda, A., Elghazaly, S., Mohammed, S. Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates. EKB Journal Management System, 2024; 5(1): 260-269. doi: 10.21608/mid.2024.255029.1711

Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of blaNDM-1 and blaKPC genes among Gram negative isolates

Microbes and Infectious Diseases
Article 28, Volume 5, Issue 1, February 2024, Page 260-269  XML PDF (524.99 K)
Document Type: Original Article
DOI: 10.21608/mid.2024.255029.1711
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Authors
Ayat H. Mohammed email 1; Ehsan A Hassan1; Mona A. Hassan1; Ahmed Reda2; Shrouk M. Elghazaly3; Shereen M. Mohammed1
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Department of Urology, Faculty of Medicine, Assiut University, Assiut, Egypt
3Intern doctor, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background:  New Delhi Metallo-b-lactamase (NDM- 1) and Klebsiella pneumoniae carbapenemase (KPC) are enzymes associated with resistance to many β- lactam agents. Their early detection is very important for controlling the spread of drug- resistant bacteria. This study aimed to evaluate the use of LOOP-mediated isothermal amplification technique (LAMP) assay for rapid and cost-effective detection of blaNDM-1 and blaKPC genes among Gram-negative bacteria in comparison with conventional PCR. Methods: A total of 156 gram-negative bacterial isolates [Escherichia coli (43), Klebsiella spp. (66), Pseudomonas spp. (47)] were screened for the presence of carbapenemases (blaNDM-1 and blaKPC) using molecular methods such as conventional PCR and LAMP assay. Results:  blaNDM1 was positive in 94/156 (60.2%) isolates and blaKPC was positive in 37/156 (23.7%) isolates by conventional PCR and LAMP. Conclusion: The LAMP technique is an excellent option for the rapid detection of blaNDM-1 and blaKPC genes. The amplification is faster and cheaper than other molecular techniques. It is easy to implement. The thermocycler is not necessary.
Keywords
LAMP; PCR; blaNDM-1; blaKPC
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