Cytokines profile and their related genotypes in COVID-19: Correlation with disease severity and outcome in Egyptian patients | ||||
| Microbes and Infectious Diseases | ||||
| Article 7, Volume 5, Issue 1, February 2024, Page 53-65 PDF (684.41 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2024.255229.1712 | ||||
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| Authors | ||||
Naglaa Said Elabd   1; Amany A. Saleh 2, 3; Asmaa Elbrolosy 4; Reda Ibrahem5; Noran T. Aboelkhair6; Mohamed Enar7; Ahmed Elesdoudy8; Marwa M. Allahouny9; Mahmoud Rizk10; Moamena S. Elhamoly 1
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| 1Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 3Medical surgical nursing Department, College of Nursing, Taibah University, KSA | ||||
| 4Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 5Public Health and Community Medicine, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 6Clinical Pathology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 7Al Mahala Elkobra fever Hospital, Al Mahala Elkobra, Egypt | ||||
| 8Chest Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 9ICU Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt | ||||
| 10Internal Medicine Department, Faculty of Medicine, Benha University, Banha, Egypt | ||||
| Abstract | ||||
| Background and Aims: COVID-19-related pulmonary inflammation is linked to elevated plasma levels of a group of proinflammatory cytokines. We aim to identify the association between IL-6 rs1800795, IL-17 rs2275913, and IL-37 rs3811046 gene polymorphisms and COVID-19 severity and prognosis. Methods: Two hundreds adult COVID-19-confirmed patients (100 patients with non-severe and 100 patients with severe or critical COVID-19) and 100 healthy individuals were enrolled in this cross-sectional study. Clinical and laboratory evaluations were performed, including liver and kidney functions, complete and differential blood counts, C-reactive protein, and D-dimer. Genotyping for IL-6 (rs1800795), IL-17 (rs2275913), and IL-37 (rs3811046) was conducted using allelic discrimination real-time PCR assay via TaqMan probes. The levels of IL-6, IL-17, and IL-37 were estimated by enzyme-linked immunosorbent assay (ELISA). Results: Serum levels of IL-6 and IL-17 were increased while IL-37 declined with ongoing COVID-19 severity. IL-6 rs1800795 genotypes and alleles did not differ significantly between the studied groups. Meanwhile, IL-17 rs2275913 GA (heterozygous) and AA (homozygous) genotypes and A allele showed significantly higher frequencies in the control group compared to those in the patients’ groups and were proposed as protective factors against COVID-19 occurrence and increased severity. Notably, IL-37 rs3811046, GT and TT variants, and T allele were more prevalent in the patients’ groups than in the control group and might be related to both disease occurrence and progression. Conclusion: Both GG genotype and G allele of IL-17 (rs2275913) and TT genotype and T allele of IL-37 (rs3811046) and their serum levels are potential risk factors for COVID-19 infection and severity, making them excellent disease management targets. | ||||
| Keywords | ||||
| COVID-19; gene polymorphism; cytokines; severity | ||||
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