Up-regulation of MiR-146a-5p and MiR-155-5p are Involved in Inflammatory Immune Dysregulation in Patients with Hashimoto’s Thyroiditis and Helicobacter Pylori Infection | ||||
| Zagazig University Medical Journal | ||||
| Articles in Press, Accepted Manuscript, Available Online from 21 January 2024 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zumj.2024.263416.3119 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Nearmeen Rashad   1; Mohamed O Wahba2; Abdelmonem Elshamy3; Marwa Abd-el monem Ateya4; manar soliman5; Hanim Magdy Abd elnour6; Amr Elhawary7
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| 1ZAGAZIG UNIVERSITY -INTERNAL MEDICINE | ||||
| 2internal medicine department -zagazig university | ||||
| 3Tropical medicine ,Zagazig university | ||||
| 4Clinical pathology department, Faculty of Medicine, Zagazig University, Zagazig, Assharqya | ||||
| 5microbiology and immunology department | ||||
| 6medical biochmestry and molecular biology department, faculty of medicine, Zagazig univerisity, Zagazig, Egypt | ||||
| 7Hepatology and Gastroenterology and endoscopy Unit-Zagazig University | ||||
| Abstract | ||||
| BBackground. Helicobacter pylori (HP) is an infection related to metabolic and autoimmune disease Hashimoto thyroiditis (HT). HP-modified microRNA (miRNA) expression leads to various disorders. We aimed to evaluate circulatory miR-146a-5p and miR-155-5p expression in HT and to investigate their correlations with the clinical profile of HT and HP infection. Methods: We enrolled 100 subjects; 50 participants as healthy control and 50 patients with HT. The diagnosis of HP- infection by HP antigen in stool and antibodies against cytotoxic-associated gene A (Cag-A). We tested serum antibodies against thyroid antigens. We used Quantitative real-time RT-PCR for the assessment of serum miR-146a-5p and miR-155-5p. Results: Among 100 studied participants, patients inf*. Concerning had significantly higher values of miR-146a-5p (3.94±0.8), compared to HT patients without HP infection (1.84±0.3) and control group (0.98±0.1), p value˂0.001*. Concerning miR-155-5p, there were statistically significant higher values in HP infection (3.61±2.2) compared to HT patients without HP infection (1.91±1.1) and control group (0.90±0.35), p value˂0.001*. There were significantly positive correlations with nausea, vomiting, heartburn, anti-TPO, anti-TG, TSH, HP antigen, and Cag-A. On the opposing, they were negatively correlated with FT3 and FT4. linear regression analyses revealed that the only variables independently associated with miR-146a-5p were anti-TPO and FT4. Whereas the only variables independent associated with miR-155-5p were anti-TPO and FT3, p value˂0.001*. Conclusion: miR-146a-5p and miR-155-5p overexpressed in patients with HT, more specifically in patients infected by HP and they were significantly correlated to gastrointestinal symptoms thyroid dysfunction, and autoimmunity. | ||||
| Keywords | ||||
| cytotoxic-associated gene A; Quantitative real-time RT-PCR; microRNAs; anti-thyroid peroxidase antibodies; H. Pylori | ||||
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