Embryo cleavage rate is enhanced by extending oocyte-cumulus cell contact: A randomized sibling oocyte study | ||||
| Journal of Reproductive Medicine and Embryology | ||||
| Article 8, Volume 1, Issue 1, January 2024, Page 48-59 PDF (410.61 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jrme.2024.263467.1006 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sherif Anis  1; Hayat Sharaf1, 2; Ahmed Abdelaziz1
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| 1Obstetrics and Gynecology Department, Faculty of Medicine Alexandria University, Egypt. | ||||
| 2Obstetrics and Gynecology Department, Faculty of Medicine Hodeidah University, Yemen. | ||||
| Abstract | ||||
| Background: Optimal embryonic developmental competence depends on proper oocyte maturation. Nuclear maturation is usually achieved in vivo and can be assessed by the time of ovum pickup; however, cytoplasmic maturation cannot be readily assessed and might be incomplete. Cumulus cells surrounding the oocyte might play a role in the oocyte cytoplasmic maturation. Objective: To investigate the effect of denudation timing after one and 2 hours on the laboratory and reproductive results in ICSI cycles. Materials and Methods: In a randomized study including 160 ICSI cycles, 2621 oocytes were randomized into two groups according to denudation timing after 1 or 2 hours of OPU. MII injection was performed immediately. Each patient had 1-2 fresh embryo transfer at day 5. Results: There is no significant difference in maturation rate between groups I and II. The total fertilization rate between the two groups was comparable at 72.6% & 73.4%, respectively (p=.675). In contrast, the cleaved embryos/ diploid zygotes in group I (94.7%) were significantly lower than those in group II (99.1%, p<.001). Also, cleaved embryos/MIl was 68.7% in group I and 72.7% in group II (p=.045). Both groups had no significant difference in the quality of embryos on days 2, 3, and 5. The available blastocysts on day 5 and the transferred grade I blastocysts were comparable. The clinically pregnant females and implantation rates were not significantly different between both groups. Conclusions: Oocyte-cumulus cell contact is important for cytoplasmic maturity. Although the impact of a higher cleavage rate after 2 hours of preincubation does not affect the clinical pregnancy rate in the fresh cycles, it may increase the probability of achieving a live birth after utilizing all cryopreserved embryos. | ||||
| Keywords | ||||
| Oocyte denudation; oocyte- cumulus cells; cytoplasmic maturation; embryo cleavage; ICSI | ||||
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