EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION
Orabi, H., El-Fakrany, E., Abdel Khalek, E., El-Kasaby, A., Sidkey, N. (2018). EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION. EKB Journal Management System, 29(Issue 2-C), 1-7. doi: 10.21608/absb.2018.33806
Hanaa M. Orabi; Esmail M. El-Fakrany; Eman S. Abdel Khalek; Aziza M. El-Kasaby; Nagwa M. Sidkey. "EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION". EKB Journal Management System, 29, Issue 2-C, 2018, 1-7. doi: 10.21608/absb.2018.33806
Orabi, H., El-Fakrany, E., Abdel Khalek, E., El-Kasaby, A., Sidkey, N. (2018). 'EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION', EKB Journal Management System, 29(Issue 2-C), pp. 1-7. doi: 10.21608/absb.2018.33806
Orabi, H., El-Fakrany, E., Abdel Khalek, E., El-Kasaby, A., Sidkey, N. EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION. EKB Journal Management System, 2018; 29(Issue 2-C): 1-7. doi: 10.21608/absb.2018.33806

EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION

Al-Azhar Bulletin of Science
Article 1, Volume 29, Issue 2-C, December 2018, Page 1-7  XML PDF (323.1 K)
Document Type: Original Article
DOI: 10.21608/absb.2018.33806
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Authors
Hanaa M. Orabi* 1; Esmail M. El-Fakrany2; Eman S. Abdel Khalek1; Aziza M. El-Kasaby1; Nagwa M. Sidkey1
1Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)
2Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt.
Abstract
L-asparaginase produced by Bacillushalotolerans OHEM18 finds broad applications due to its hydrolysis activity so used as therapeutic agents in combination with additional medications in the cure of acute lymphoblastic leukemia and also in food processing. In the present study, L-asparaginase production was optimizedand the optimum factors for production under submerged fermentation (SMF) of the asparaginase were pH 7.0 after 28h using glucose and asparagine as carbon and nitrogen sources, respectively while bagasse was the best inducer for L-asparaginase production under SSF.
Keywords
L-asparaginase; enzyme production; submerged fermentation and solid state fermentation
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