Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series
Guiziry, D., Adel, N., Faris, M., Fathy, E., Abo Ali, A., Maghraby, H., Elmahdy, M., Maghraby*, H. (2024). Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series. EKB Journal Management System, 1(1), 60-65. doi: 10.21608/jrme.2024.264068.1007
Dalal Guiziry; Nehal Adel; Mohammed Faris; Esraa Fathy; Ashraf Abo Ali; Heba Maghraby; Mohamed Elmahdy; Hassan Maghraby*. "Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series". EKB Journal Management System, 1, 1, 2024, 60-65. doi: 10.21608/jrme.2024.264068.1007
Guiziry, D., Adel, N., Faris, M., Fathy, E., Abo Ali, A., Maghraby, H., Elmahdy, M., Maghraby*, H. (2024). 'Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series', EKB Journal Management System, 1(1), pp. 60-65. doi: 10.21608/jrme.2024.264068.1007
Guiziry, D., Adel, N., Faris, M., Fathy, E., Abo Ali, A., Maghraby, H., Elmahdy, M., Maghraby*, H. Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series. EKB Journal Management System, 2024; 1(1): 60-65. doi: 10.21608/jrme.2024.264068.1007

Double Ionophore Activation (DIA) technique in cases of severe male factor: Case Series

Journal of Reproductive Medicine and Embryology
Article 9, Volume 1, Issue 1, January 2024, Page 60-65  XML PDF (279.88 K)
Document Type: Case Series
DOI: 10.21608/jrme.2024.264068.1007
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Authors
Dalal Guiziry1, 2, 3; Nehal Adel email orcid 3, 4; Mohammed Faris2, 5; Esraa Fathy2; Ashraf Abo Ali3, 4; Heba Maghraby2, 3; Mohamed Elmahdy3, 6; Hassan Maghraby*2, 3, 6
1Clinical Pathology Department, Faculty of Faculty of Medicine Alexandria University, Egypt.
2Dar Alteb Infertility Center, Alexandria, Egypt.
3Egyptian Foundation of Reproductive Medicine and Embryology (EFRE), Egypt.
4Madina ICSI Fertility Center, Madina women's hospital, Alexandria, Egypt.
5Obstetrics and Gynecology Department, Faculty of Medicine, Ain Shams University, Egypt
6Obstetrics and Gynecology Department, Faculty of Medicine, Alexandria University, Egypt.
Abstract
Background: Sperm binding or injection into human oocytes induces calcium oscillations resulting in oocyte activation, fertilization, and subsequent embryonic development.  In severe male factor, sperm may fail to activate oocytes with subsequent ICSI failure.  Artificial oocyte activation with calcium ionophore can induce calcium influx with improved oocyte activation and development.
Objective: The current case series evaluated a unique technique of double ionophore activation (DIA) in severe male factor without previous ICSI failure.
Materials and Methods: Case series of 17 infertile couples with severe male factors undergoing ICSI. The injected oocytes were incubated in calcium ionophore twice with this new protocol for artificial oocyte activation.
Results: Fertilization, cleavage, and blastocyst rates were 71.4%, 100%, and 73.3% respectively.  The pregnancy rate for fresh transfer was 9/13 (69.2%) and the pregnancy rate for FET was 4/6 (66.7%).
Conclusion: DIA protocol may improve oocyte activation in severe male factor and a well-designed prospective study is needed.
Keywords
Oocyte; oligoathenoteratozoospermia; fertilization; ICSI; Blastocyst rate
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