Dynamics of Haemonchus Contortus Coproantigen Appearance in Feces of Experimentally Infected Sheep

Shalaby, Hatem; Kandil, Omnia; Hendawy, Seham; Elsawy, Bassma S M; Ashry, Heba M Z; El-Namaky, Amira; Ata, Emad B

doi:10.21608/ejvs.2024.251684.1693

	Dynamics of Haemonchus Contortus Coproantigen Appearance in Feces of Experimentally Infected Sheep
Egyptian Journal of Veterinary Sciences
Volume 55, Issue 5, September and October 2024, Page 1307-1314 PDF (892.62 K)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.251684.1693
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Authors
Hatem Shalaby¹; Omnia Kandil²; Seham Hendawy ³; Bassma S M Elsawy⁴; Heba M Z Ashry⁵; Amira El-Namaky ⁶; Emad B Ata ⁷
¹parasitology and animal diseases department nvetrinary institute ,NRC
²Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt,
³Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Cairo, Egypt.
⁴parasitology department
⁵Parasitology,department
⁶Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, Cairo, Egypt.
⁷Parasitology Dep
Abstract
Haemonchosis is one of the most important parasitic diseases due to its economic effect on the productivity of small ruminants. Accordingly, there is a significant need for the development of accurate immunological assays for early diagnosis. Little is known about coproantigen’s dynamics in the feces of Haemonchus contortus (H. contortus)- infected sheep before egg shedding. In this study, two immunodiagnostic techniques were used: antigen capture-Enzyme Linked Immunosorbent Assay (ELISA) and Enzyme-Linked Immunoelectrotransfer Blot (EITB). At 5-8 days post infection (DPI), antigen capture- ELISA detected H. contortus coproantigen in feces of the experimentally infected lambs. Diagnostic detection of the antigen using capture ELISA was recorded four days earlier than detection with indirect ELISA. EITB was more accurate in the early detection of coproantigen in fecal supernatants at 2^nd DPI. Two specific polypeptide bands of 42 and 126 KDa strongly reacted with the sera of experimentally infected lambs. Additionally, two specific polypeptide bands of H. contortus coproantigen with molecular weights of 54 and 59 KDa might be considered a reliable parameter in detecting the early phase of infection before egg shedding. Reactivity against these bands was observed beginning with the 8^th DPI and lasting until the experiment ended. In conclusion, H. contortus coproantigen could be used as a diagnostic antigen in sheep to detect haemonchosis during the prepatent period.
Keywords
Haemonchus contortus; coproantigen; capture ELISA; EITB; sheep


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