Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV
Abo- El Wafa, H., Mohammad, M., Sayed, S., Abdel Allah, A., Ahmed, M., Mahmoud, A. (2017). Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV. EKB Journal Management System, 21(3), 11-16. doi: 10.21608/smj.2017.34036
Hasnaa Ahmed Abo- El Wafa; Mohammad Abd Allah Mohammad; Sherif Abdel Aziz Sayed; Amany Abbas Abdel Allah; Mustafa Adel Ahmed; Al Zahra Nasser Mahmoud. "Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV". EKB Journal Management System, 21, 3, 2017, 11-16. doi: 10.21608/smj.2017.34036
Abo- El Wafa, H., Mohammad, M., Sayed, S., Abdel Allah, A., Ahmed, M., Mahmoud, A. (2017). 'Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV', EKB Journal Management System, 21(3), pp. 11-16. doi: 10.21608/smj.2017.34036
Abo- El Wafa, H., Mohammad, M., Sayed, S., Abdel Allah, A., Ahmed, M., Mahmoud, A. Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV. EKB Journal Management System, 2017; 21(3): 11-16. doi: 10.21608/smj.2017.34036

Influence of Temperature And Storage Duration on Measurement of Prothrombin TimeV

Sohag Medical Journal
Article 1, Volume 21, Issue 3, October 2017, Page 11-16  XML PDF (96.3 K)
Document Type: Original Article
DOI: 10.21608/smj.2017.34036
View on SCiNiTO View on SCiNiTO
Authors
Hasnaa Ahmed Abo- El Wafa; Mohammad Abd Allah Mohammad; Sherif Abdel Aziz Sayed; Amany Abbas Abdel Allah; Mustafa Adel Ahmed; Al Zahra Nasser Mahmoud
Department of Clinical Pathology,Faculty of Medicine, Sohag University.
Abstract
Objectives:toinvestigate the sample stabilitywith regard to the temperatureand the storage time and to detect influence of temperature and storage duration on Prothrombin time in citrated plasma.
Patients and Method:This study was carried out in the period between April, 2016 and March, 2017 in the department of clinical pathology, Sohag University Hospital, Sohag, Egypt, during the study period whole blood samples were collected from groups of patients: "43"apparently normal healthy (not receiving anticoagulant) as control (group A) and"43"receiving anticoagulant (17 on warfarin and 26 on heparin) (group B).Each sample of each group will be analyzed forProthrombin time immediately and after 4hrs, 8hrs, and 24hrs of storage.Also each sample will be tested at room temperature (18-22 °C) and at refrigerator temperature (4 °C).
Results andConclusion:samples for Prothrombin time were acceptable for analyses up to 24 h in those not receiving anticoagulant and up to 8 h in those receiving anticoagulant.
Supplementary Files
References
  1. Lippi G., Guidi G.C., Mattiuzzi C. &Plebani M. (2006) Preanalytical variability: the dark side of the moon in laboratory testing. Clinical Chemistry and Laboratory Medicine 44, 358–365.
  2. Palmer RN and Gralnick HR (1985): Inhibition of cold-promoted activation of the prothrombin time. Studies of new siliconised borosilicate collection tubes in normals and patients receiving warfarin.Am J Clin Path 83: 492–494.
  3. Adcock DM, Hoefner DM, Kottke-Marchant K, Marlar RA, Szamosi DI, Warunek  DJ. (2008). Collection, transport, and processing of blood samples for testing plasma-based coagulation assays and molecular hemostasis assays. Approved guideline: 5th ed. (H21-A5). Wayne, PA: Clinical and Laboratory Standards Institute.
  4. Mohammed Saghir, S. A., Al-Hassan, F. M., Alsalahi, O. S., Abdul Manaf, F. S. &Baqir, H. S. (2012). Optimization of the storage conditions for coagulation screening tests. J Coll Physicians Surg Pak 22, 294–297.
  5. Brigden M.L., Graydon C., McLeod B. &Lesperance M. (1997) Prothrombin time determination. The lack of need for a discard tube and 24-h stability. American Journal of Clinical Pathology 108, 422–426.
  6. NCCLS (2003).National Committee for Clinical Laboratory Standards.Collection, Transport and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays.Approved Guideline H21-A4, 3rd edn.NCCLS, Wayne, PA
  7. L.V. Rao, A.O. Okorodudu, J.R. Petersen and M.T. Elghetany., (2000).ClinicaChimicaActa300 (P 13 –21).
  8. Adcock D, Kressin D, Marlar RA.(1998).The effect of time and temperature variables on routine coagulation tests. Blood CoagulFibrinol; 9:463–470.
  9. Wang, X., Ma, J. Z. H. &Hao, Z. L. (2002). Influence of storage time of PT and APTT at different temperature. Journal of Hebei Medical University 23, 108–109.
  10. Salvagno GL, Lippi G, Montagnana M, Franchini M, Poli G, Guidi GC(2009). Influence of temperature and time before centrifugation of specimens for routine coagulation testing. Int J Lab Hematol; 31:462– 467.
  11. Zu¨rcher M, Sulzer I, Barizzi G, La¨mmle B, Alberio L (2008). Stability of coagulation assays performed in plasma from citrated whole blood transported at ambient temperature. ThrombHaemost; 99:416–426
  12. Wang B, Guo W, Pan B. Influence of storage time at room temperature on routine coagulation tests. Chin J Lab Med2011;34:595–7.
  13. Koepke JA, Rodgers JL, Ollivier MJ.(1975). Pre-instrumental variables in coagulation testing. Am J Clin Pathol;64:591–6.
  14. Neofotistos D, Oropeza M, HsinTsao C.(1998) Stability of plasma for add on PT and aPTT tests. Am J Clin Pathol;109:758–63.
  15. Kemkes-Matthes B, Fischer R, Peetz D (2011).Influence of 8 and 24-h storage of whole blood at ambient temperature on prothrombin time, activated partial thromboplastin time, fibrinogen, thrombin time, antithrombin and D-dimer. Blood CoagulFibrinolysis;22:215–20
  16. Van Geest-Daalderop JH, Mulder AB, Boonman-de Winter LJ, Hoekstra MM, van den Besselaar AM.( 2005). Preanalytical variables and off-site blood collection: influences on the results of the prothrombin time/international normalized ratio test and implications for monitoring of oral anticoagulant therapy. Clin Chem;51:561–8.
 

Statistics
Article View: 430
PDF Download: 302