Chronic kisspeptin-10 ameliorates osteoporotic changes in orchidectomized male albino rats: involvement of Bone morphogenic protein-2 and Wnt/β-catenin signaling pathways | ||||
Zagazig University Medical Journal | ||||
Articles in Press, Accepted Manuscript, Available Online from 08 February 2024 | ||||
Document Type: Original Article | ||||
DOI: 10.21608/zumj.2024.264538.3128 | ||||
View on SCiNiTO | ||||
Authors | ||||
Marwa Abdelaziz Habib 1; Heba Abdelgeleel 2; Reham Ebrahim 3 | ||||
1Physiology department, Faculty of Medicine, Zagazig University, Egypt | ||||
2Pathology department faculty of Medicine, Zagazig University | ||||
3Physiology department faculty of medicine Zagazig university Zagazig Sharkia | ||||
Abstract | ||||
Osteoporosis is a serious worldwide skeletal illness manifested by diminished bone density with increased susceptibility to fractures and increases with aging. Kisspeptin is a hypothalamic neuropeptide that regulates the gonadal hormones and was suggested to have a positive effect on bone metabolism. Material & Methods: 30 male rats were divided equally in to three groups: Sham, orcidectomized (ORX), and kisspeptin-10 (kiss)-treated groups. Blood, urine, and femoral bone samples were taken for biochemical, histopathological, and gene studies. Results: ORX rats showed increased serum levels of alkaline phosphatase (ALP), osteocalcin, RANKL, Osteopetrogenin (OPG), RANKL/ OPG ratio, tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6(, while decreased serum OPG level and bone minerals associated with disruption of bone architecture compared with Sham group. Chronic treatment with kisspeptin-10 significantly enhanced bone architecture, increased bone minerals, improved RANKL/OPG ratio, up-regulated mRNA expression levels of OPG, bone morphogenic protein 2 (BMP-2), Wnt3a, and β-catenin as compared to untreated ORX rats. Conclusion: Chronic treatment with kisspeptin-10 enhances osteogenesis and reduces bone resorption in ORX rats via modulating RANKL/OPG ratio, BMP-2, and Wnt/β-catenin signaling pathways. So, the present study recommends kisspeptin-10 as a promising novel therapeutic agent for age-related osteoporosis in men. | ||||
Keywords | ||||
Osteoporosis, Kisspeptin, BMP-2, Wnt/β -catenin pathway | ||||
Statistics Article View: 100 |
||||