Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats | ||||
| Egyptian Journal of Botany | ||||
| Article 1, Volume 56, Issue 1, September 2016, Page 1-14 PDF (787.22 K) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2016.341 | ||||
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| Abstract | ||||
| HE VARIABILITY of grain storage-proteins of twelve wheat …..genotypes under stress (Siwa Oasis) and normal (Ashmon) habitats were analyzed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). It was clear that small differences were found in the SDS-PAGE analysis of wheat endosperm proteins among the studied genotypes. The defense isozymes [Peroxidase (Px), Polyphenol oxidase (PPO) and Malate dehydrogenase (Mdh)] were detected and their results concluded a prominent role, either directly or indirectly in stress responses. High polymorphisms were observed in most genotypes forMdh isozymes at Siwa habitat, meanwhile Px isozymes were decreased. Misr-1 genotype attained a higher level of Px isozymes under stress habitat as a trait to tolerance response. The changes of PPO isozymes showed a good correlation with the abiotic stress tolerance of wheat genotypes. Thus, PPO increased in Nesr, S8/17, ACSAD-2, ACSAD-3 and NBL genotypes under stress condition (Siwa habitat). | ||||
| Keywords | ||||
| Wheat genotypes; isozymes Siwa Oasis and Ashmon habitats; Protein profiling; peroxidase; Polyphenol oxidase and Malate dehydrogenase | ||||
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