Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus
Younes, A., Y. Gaafar, A., S. Awad1, E., S. Soliman, W., B. Salman, M., F. Eida3, M., A. Mohamed, L. (2024). Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus. EKB Journal Management System, 55(6), 1553-1559. doi: 10.21608/ejvs.2024.253308.1701
Abdelgayed M. Younes; Alkhateib Y. Gaafar; Elham S. Awad1; Waleed S. Soliman; Marwa B. Salman; Mohamed F. Eida3; Laila A. Mohamed. "Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus". EKB Journal Management System, 55, 6, 2024, 1553-1559. doi: 10.21608/ejvs.2024.253308.1701
Younes, A., Y. Gaafar, A., S. Awad1, E., S. Soliman, W., B. Salman, M., F. Eida3, M., A. Mohamed, L. (2024). 'Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus', EKB Journal Management System, 55(6), pp. 1553-1559. doi: 10.21608/ejvs.2024.253308.1701
Younes, A., Y. Gaafar, A., S. Awad1, E., S. Soliman, W., B. Salman, M., F. Eida3, M., A. Mohamed, L. Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus. EKB Journal Management System, 2024; 55(6): 1553-1559. doi: 10.21608/ejvs.2024.253308.1701

Incidence of aac(6′)-Ib-cr Mediated by Class 1 Integrons in Aeromonas Species Isolated from Diseased Oreochromis niloticus

Egyptian Journal of Veterinary Sciences
Volume 55, Issue 6, November and December 2024, Page 1553-1559  XML PDF (934.45 K)
Document Type: Original Article
DOI: 10.21608/ejvs.2024.253308.1701
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Authors
Abdelgayed M. Younes email orcid 1; Alkhateib Y. Gaafar1; Elham S. Awad11; Waleed S. Soliman1; Marwa B. Salman2; Mohamed F. Eida33; Laila A. Mohamed1
1Hydrobiology Department, Veterinary Division, National Research Centre, Dokki, Cairo, 12622, Egypt
2Department of zoonotic diseases, Veterinary Division, National Research Centre, Dokki, Cairo, 12622, Egypt
3Microbiology Department, Agriculture Division, National Research Centre, Dokki, Cairo, 12622, Egypt
Abstract
The virulent clonal populations of Aeromonas spp. are recognized as the etiological agent of motile Aeromonas septicemia (MAS) outbreaks in aquaculture. This study aimed to identify Aeromonas spp. isolated from diseased Oreochromis niloticus, evaluate their virulence factors, and assess their resistance to antimicrobial agents. Species identification was conducted through analysis of the gyrB genes. Antibiotic resistance phenotypes and genotypes (blaTEM, blaCTX-M, aac(6′)-Ib-cr, and  intI1) were determined. The results indicated that 44,4% of the isolates belonged to A. veronii, 38.9% to A. sobria, and 16.7% to A. hydrophila. PCR analysis revealed the presence of blaTEM, aac(6′)-Ib-cr, and intI1 genes among 100%, 77.8%, and 83.3% of the isolates, respectively, while blaCTX-M was not detected. In conclusion, the detection of the aac(6′)-Ib-cr gene in aquatic environments can serve as a significant indicator of multi-drug resistance (MDR). The presence of MDR, particularly mediated by integrons in Aeromonas, raises serious concerns regarding potential health risks. Moreover, the emergence of MDR complicates the efficacy of therapeutic treatments for infections. Therefore, adopting a comprehensive One Health approach becomes imperative to effectively control the spread of MDR and mitigate its impact on public health.
Keywords
Aeromonas spp; Oreochromis niloticus; virulence genes; antimicrobial resistance genes
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