METABOLITES PROFILING AND IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES OF THREE DIFFERENT MARINE SPONGES METHANOLIC EXTRACTS | ||||
| Minia Journal of Agricultural Research and Development | ||||
| Volume 44, Issue 1, March 2024, Page 73-96 PDF (893.55 K) | ||||
| DOI: 10.21608/mjard.2024.341647 | ||||
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| Abstract | ||||
| In the current study, three different marine sponges methanolic extracts Clathria arbuscula (SR), Crella cyathophora (S), and Xenia macrspiculata (CS) were collected from the Red Sea in Hurghada and examined for their metabolite profiles, antioxidant, and anticancer properties. Flavonoid, total phenolic , (2,2-diphenyl-1-picrylhydrazyl )DPPH, and ( Ferric Reducing Antioxidant Power)FRAP tests for antioxidants were used to identify the different types of marine sponges methanolic extracts. The anticancer action of the cancer cells MDA-MB 231, HUH-7, and NB4-APL was dose- and extract-dependent. Total phenolic content (TPC) of Xenia macrspiculata methanolic extract has been found to be higher than that of Clathria arbuscula methanolic extract and Crella cyathophora methanolic extract . While the total flavonoid content (TFC) of Xenia macrspiculata methanolic extract has been shown to be higher than that of Clathria arbuscula methanolic extract and Crella cyathophora methanolic extract. Different levels of FRAP and DPPH activity have been found in all methanolic extracts of marine sponges. It has been worked the UPLC-TOF-MS analysis for the three different extracts. A total of 1,054 metabolites were found in the metabolome analysis. It was divided the primary groups of chemicals were and peptides, amino acids ,fatty acids and conjugates, organic dicarboxylic acids, purines, benzoic acids, indoles, pyrimidines, benzenes, phenylacetic acids, and pyridines compounds. The multivariate statistical analysis identified 32 metabolites, including 2-Chloro-N, N-dimethyl- 3- oxobutanamide, 4-Methyl-2-oxo-pentanoic acid, ,6- Dihydrouracil, 3-Hydroxy-3-methylglutaric acid, (E)-3-hydroxy-2- (2-hydroxypentadecanoylamino) dec-4-ene-1-sulfonic acid, Cysteine sulfinic acid,10-Hydroxydecanoic acid, 1-Aminocyclopropane-1- | ||||
| Keywords | ||||
| Antioxidant, Anticancer, Biomarker compounds, Clathria arbuscula; Crella cyathophora; Xenia macrspiculata; Untargeted metabolomics | ||||
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