The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel
Elnady, I., Elsalini, O., Abdel-Khalek, A., Khalil, W., Shamiah, S. (2023). The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel. EKB Journal Management System, 48(2), 155-162. doi: 10.21608/ajar.2024.216837.1169
Ismail Abdelraouf Elnady; O. A. Elsalini; A. E. Abdel-Khalek; W. A. Khalil; S. M. Shamiah. "The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel". EKB Journal Management System, 48, 2, 2023, 155-162. doi: 10.21608/ajar.2024.216837.1169
Elnady, I., Elsalini, O., Abdel-Khalek, A., Khalil, W., Shamiah, S. (2023). 'The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel', EKB Journal Management System, 48(2), pp. 155-162. doi: 10.21608/ajar.2024.216837.1169
Elnady, I., Elsalini, O., Abdel-Khalek, A., Khalil, W., Shamiah, S. The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel. EKB Journal Management System, 2023; 48(2): 155-162. doi: 10.21608/ajar.2024.216837.1169

The Influence of Oocyte Diameter and Culture Media Type on in vitro Nuclear Maturation in Camel

Al-Azhar Journal of Agricultural Research
Volume 48, Issue 2, December 2023, Page 155-162  XML PDF (853.54 K)
Document Type: Original Article
DOI: 10.21608/ajar.2024.216837.1169
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Authors
Ismail Abdelraouf Elnady email orcid 1; O. A. Elsalini2; A. E. Abdel-Khalek3; W. A. Khalil3; S. M. Shamiah4
1Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
2Zoology Department, Faculty of Science, Benghazi University, Libya.
3Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt.
4Biotechnology Department, Animal Production Research Institute, Agriculture Research Center, Egypt.
Abstract
This study aimed to determine the influence of oocyte diameter and culture medium (tissue culture medium (TCM-199) and minimal essential medium (MEM)) on in vitro nuclear maturation in camel (Camelus dromedarius). The recovered oocytes (523 oocytes) were classified into three diameter categories: including ≤120, 120-150, and ≥150 µm. The results revealed that Oocytes matured in TCM-199 had a significantly (p<0.05) higher percentage of oocytes reaching the metaphase II (MII) (46.34%) than oocytes matured in MEM medium (38.63%). The percentage of oocytes arrested at MII was significantly (p<0.05) higher for oocytes with a diameter of ≥150µm (53.25%), moderate for 120-150µm (43.36%) and lowest for ≤120µm (24.16%) oocytes, regardless of the type of maturation medium. The interaction between culture medium and oocyte diameter was insignificant, except for interaction between TCM-199 medium and ≥ 150 μm of oocyte diameter, reflecting a higher percentage of oocytes arrested at MII (58.88%). In conclusion, oocytes with a diameter of ≥ 150 µm matured in TCM-199 medium have the potential to improve the in vitro maturation of camel oocytes.
Keywords
camel; oocyte diameter; culture medium type; nuclear maturation
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