Antibacterial properties of some medicinal plant extracts against pathogenic bacteria forming biofilms: Bioactive compounds identification from potential extract and cytotoxicity activity | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 67, Issue 9, September 2024, Page 111-126 PDF (1.86 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2024.262509.9179 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Yasmine N Elsawy  1; Hemmat Mohammed Abd-Elhady2; Khadiga Ahmed Abou-Taleb 3; Rania Farouk Ahmed4
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| 1faculty of agriculture Ain- shams university | ||||
| 2Emeritus Prof. of Agric. Microbiology, Dept. of Agric. Microbiology, Fac. of Agriculture, Ain shams Univ. | ||||
| 3Prof. of Agric. Microbiology, Dept. of Agric. Microbiology, Fac. of Agriculture, Ain shams Univ. | ||||
| 4Assoc. Prof. of Agric. Microbiology, Dept. of Agric. Microbiology, Fac. of Agriculture, Ain shams Univ. | ||||
| Abstract | ||||
| Medicinal plant extracts are excellent resources of bioactive compounds with perfect antibacterial and antioxidant activity. Phytochemicals are an exciting source of natural pharmaceuticals that are applied instead of synthetic ones and control the growth of multidrug resistant bacteria. About 41.66% of Gram-positive bacteria were detected as biofilm-forming bacteria, while 36.66% were classified as biofilm-forming bacteria belonging to the Enterobacteriaceae family. Marigold aquatic extract scored the highest inhibition zone with 7.4 to 13.7%, followed by Moringa with 7.4 to 12.6% against all G+ve bacteria. While the tested Enterobacteriaceae G-ve shows higher resistance to the effect of aquatic extracts. The inhibition zone of ethanolic extracts reached 8.4 to 26.3% for Sage, followed by Cinnamon and marigold, which scored 9.5 to 22% and 7.4 to 21%, respectively. The extracts yield percentages ranged from 11.24 to 12.8%. The clear zone diameter recoded by ethanolic Sage extract against G+ve and G-ve in descending order was Salmonella Typhimurium ATCC25566> Listeria monocytogenes ATCC7646> Bacillus cereus ATCC11778> Staphylococcus aureus ATCC5638. The bacterial activity of all ethanolic plant extracts achieved the greatest clear zone against tested bacteria, whereas chloroform and methanol recorded the weakest solvents. According to examination using gas chromatography and mass spectrometry, ethanolic Sage extract contains 27 different phytochemical components, with 13-Docosenamide, (Z)- having the highest area with a percentage 14.4%. At doses up to 100 µg/ml, the ethanolic Sage extract showed no cytotoxicity effect to the typical Vero cell line, whereas the IC50 value reached 190.67±4.23µg/ml. | ||||
| Keywords | ||||
| Antibacterial activities; Cytotoxicity effect; GC/MS analysis; Medicinal plant extract; Solvents extraction. | ||||
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