EFFECT OF NANO SELENIUM SUPPLEMENTATION ON SEMEN CHARACTERISTICS STORED AT COOLTEMPERATURE | ||||
| Journal of Productivity and Development | ||||
| Article 4, Volume 28, Issue 4, October 2023, Page 245-261 PDF (666.66 K) | ||||
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| Authors | ||||
Hassan El kholy1; El-shinawy El-Siefy1; Hassan El Kelawy  2; Nagy El Gaafary3
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| 1Biotechnology Research Department, Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Egypt. | ||||
| 2Department of Animal and Poultry Production, Faculty of Technology & Development, Zagazig University, Zagazig, Egypt. | ||||
| 3Department of Animal and Poultry Production, Faculty of Technology & Development, Zagazig University, Zagazig, Egypt. | ||||
| Abstract | ||||
| Semen was collected once a week from four sexually mature buffalo bulls raised at International Livestock Management Training Center (ILMTC), Sakha, belonging to the Animal Production Research Institute, to evaluate the effect of addition different concentrations of selenium Nano-particles (SeNPs) on sperm motility, livability, abnormality, plasma membrane and acrosome integrity in semen stored at 5°C for different storage periods. Only ejaculates with mass motility of 70% or more were pooled for each collection day. The collected semen was diluted with Tris-extender and held in a water bath at 37°C, then divided into six portions. Different concentrations of SeNPs were added to the six diluted portions of semen (0 (control), 25, 0.5, 0.75, 1 and 1.25µg/mL /mLTris extender). Thereafter, all portion of semen were stored at 5°C (refrigerator temperature) for 0, 6, 24, 48, 72 and 96 h periods. Percentages of progressive motility, livability, abnormality, plasma membrane and acrosome integrity of spermatozoa were determined at various storage times. Results revealed that addition SeNPs improved all semen characteristics during different storage periods compared to control but the differences during the periods of 0, 24 and 48 h of storage were not significant. While the differences were highly (P<0.05) significant among the extenders supplemented with SeNPs compared with control during the periods of 72 and 96 h of storage. Sperm motility, livability, plasma membrane and acrosome integrity decreased (P<0.05) by increasing storage period. However, sperm abnormality was increased affected by storage period,. Semen supplemented with 1.25 µg SeNPs was the highest sperm motility, livability, plasma membrane and acrosome integrity. Conclusively, the present results suggested the possibility of storage buffalo bulls semen at 5°C for 72 h with maintenance of sperm motility, livability, plasma membrane and acrosome integrity percentages above 40% in semen supplemented with 1.25µg/mL /mLSeNPs. Accordingly, it can be used for artificial insemination. | ||||
| Keywords | ||||
| buffalo sperm; SeNPs; storage; semen characteristics | ||||
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